| |  Online-Ressource |
|---|
| Verfasst von: | Schuetzmann, Daniel [VerfasserIn]  |
|---|
| | Walter, Carolin [VerfasserIn] |
|---|
| | Riel, Boet van [VerfasserIn] |
|---|
| | Kruse, Sabrina [VerfasserIn] |
|---|
| | König, Thorsten [VerfasserIn] |
|---|
| | Erdmann, Tabea [VerfasserIn] |
|---|
| | Tönges, Alexander [VerfasserIn] |
|---|
| | Bindels, Eric [VerfasserIn] |
|---|
| | Weilemann, Andre [VerfasserIn] |
|---|
| | Gebhard, Claudia [VerfasserIn] |
|---|
| | Wethmar, Klaus [VerfasserIn] |
|---|
| | Perrod, Chiara [VerfasserIn] |
|---|
| | Minderjahn, Julia [VerfasserIn] |
|---|
| | Rehli, Michael [VerfasserIn] |
|---|
| | Delwel, Ruud [VerfasserIn] |
|---|
| | Lenz, Georg [VerfasserIn] |
|---|
| | Gröschel, Stefan [VerfasserIn] |
|---|
| | Dugas, Martin [VerfasserIn] |
|---|
| | Rosenbauer, Frank [VerfasserIn] |
|---|
| Titel: | Temporal autoregulation during human PU.1 locus SubTAD formation |
|---|
| Verf.angabe: | Daniel Schuetzmann, Carolin Walter, Boet van Riel, Sabrina Kruse, Thorsten König, Tabea Erdmann, Alexander Tönges, Eric Bindels, Andre Weilemann, Claudia Gebhard, Klaus Wethmar, Chiara Perrod, Julia Minderjahn, Michael Rehli, Ruud Delwel, Georg Lenz, Stefan Gröschel, Martin Dugas, and Frank Rosenbauer |
|---|
| E-Jahr: | 2018 |
|---|
| Jahr: | October 12, 2018 |
|---|
| Umfang: | 13 S. |
|---|
| Fussnoten: | Gesehen am 27.08.219 |
|---|
| Titel Quelle: | Enthalten in: Blood |
|---|
| Ort Quelle: | Washington, DC : American Society of Hematology, 1946 |
|---|
| Jahr Quelle: | 2018 |
|---|
| Band/Heft Quelle: | 132(2018), 25, Seite 2643-2655 |
|---|
| ISSN Quelle: | 1528-0020 |
|---|
| Abstract: | Visual Abstract - <img class="highwire-fragment fragment-image" alt="Figure1" src="http://www.bloodjournal.org/content/bloodjournal/132/25/2643/F1.medium.gif" width="440" height="222"/>Download figureOpen in new tabDownload powerpoint - - Epigenetic control of gene expression occurs within discrete spatial chromosomal units called topologically associating domains (TADs), but the exact spatial requirements of most genes are unknown; this is of particular interest for genes involved in cancer. We therefore applied high-resolution chromosomal conformation capture sequencing to map the three-dimensional (3D) organization of the human locus encoding the key myeloid transcription factor PU.1 in healthy monocytes and acute myeloid leukemia (AML) cells. We identified a dynamic ∼75-kb unit (SubTAD) as the genomic region in which spatial interactions between PU.1 gene regulatory elements occur during myeloid differentiation and are interrupted in AML. Within this SubTAD, proper initiation of the spatial chromosomal interactions requires PU.1 autoregulation and recruitment of the chromatin-adaptor protein LDB1 (LIM domain-binding protein 1). However, once these spatial interactions have occurred, LDB1 stabilizes them independently of PU.1 autoregulation. Thus, our data support that PU.1 autoregulates its expression in a “hit-and-run” manner by initiating stable chromosomal loops that result in a transcriptionally active chromatin architecture. |
|---|
| DOI: | doi:10.1182/blood-2018-02-834721 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1182/blood-2018-02-834721 |
|---|
| | Volltext: http://www.bloodjournal.org/content/132/25/2643 |
|---|
| | DOI: https://doi.org/10.1182/blood-2018-02-834721 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| K10plus-PPN: | 167217810X |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Temporal autoregulation during human PU.1 locus SubTAD formation / Schuetzmann, Daniel [VerfasserIn]; October 12, 2018 (Online-Ressource)
