Structure of the nuclear exosome captured on a maturing preribosome

• Verfasst von: Schuller, Jan Michael [VerfasserIn]
• Verfasst von: Falk, Sebastian [VerfasserIn]
• Verfasst von: Fromm, Lisa [VerfasserIn]
• Verfasst von: Hurt, Ed [VerfasserIn]
• Verfasst von: Conti, Elena [VerfasserIn]
• Titel: Structure of the nuclear exosome captured on a maturing preribosome
• Verf.angabe: Jan Michael Schuller, Sebastian Falk, Lisa Fromm, Ed Hurt, Elena Conti
• E-Jahr: 2018
• Jahr: March 8, 2018
• Umfang: 4 S.
• Fussnoten: Gesehen am 27.08.2019
• Titel Quelle: Enthalten in: Science
• Ort Quelle: Washington, DC [u.a.] : American Association for the Advancement of Science, 1880
• Jahr Quelle: 2018
• Band/Heft Quelle: 360(2018), 6385, Seite 219-222
• ISSN Quelle: 1095-9203
• DOI: doi:10.1126/science.aar5428
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1672192633

Abstract

The RNA exosome captured in action - The RNA exosome, a major RNA degradation machine, processes ribosomal RNA (rRNA) precursors and is directly coupled to the protein synthesis machine, the ribosome. Using cryo-electron microscopy, Schuller et al. investigated the structure of the precursor large ribosomal subunit from yeast with unprocessed rRNA in complex with the RNA exosome. The structure captures a snapshot of two molecular machines transiently interacting and explains how the RNA exosome acts on an authentic physiological substrate and remodels the large subunit during ribosome maturation. - Science, this issue p. 219 - The RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo-electron microscopy to visualize the yeast nuclear exosome holocomplex captured on a precursor large ribosomal subunit (pre-60S) during 7S-to-5.8S rRNA processing. The cofactors of the nuclear exosome are sandwiched between the ribonuclease core complex (Exo-10) and the remodeled “foot” structure of the pre-60S particle, which harbors the 5.8S rRNA precursor. The exosome-associated helicase Mtr4 recognizes the preribosomal substrate by docking to specific sites on the 25S rRNA, captures the 3′ extension of the 5.8S rRNA, and channels it toward Exo-10. The structure elucidates how the exosome forms a structural and functional unit together with its massive pre-60S substrate to process rRNA during ribosome maturation. - The exosome forms a single structural and functional unit with its preribosomal substrate to catalyze ribosomal RNA processing and ribosome maturation. - The exosome forms a single structural and functional unit with its preribosomal substrate to catalyze ribosomal RNA processing and ribosome maturation.