Differences in self-recognition between secreted antibody and membrane-bound B cell antigen receptor

• Verfasst von: Iype, Joseena Mariam [VerfasserIn]
• Verfasst von: Wardemann, Hedda [VerfasserIn]
• Titel: Differences in self-recognition between secreted antibody and membrane-bound B cell antigen receptor
• Verf.angabe: Joseena Iype, Moumita Datta, Ahmad Khadour, Rudolf Übelhart, Antonella Nicolò, Tim Rollenske, Marcus Dühren-von Minden, Hedda Wardemann, Palash C. Maity and Hassan Jumaa
• E-Jahr: 2019
• Jahr: 25 January 2019
• Umfang: 11 S.
• Fussnoten: Gesehen am 04.09.2019
• Titel Quelle: Enthalten in: The journal of immunology
• Ort Quelle: Bethesda, Md. : Soc., 1916
• Jahr Quelle: 2019
• Band/Heft Quelle: 202(2019), 5, Seite 1417-1427
• ISSN Quelle: 1550-6606
• DOI: doi:10.4049/jimmunol.1800690
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1675867607

Abstract

The random gene segment rearrangement during B cell development ensures Ab repertoire diversity. Because this process might generate autoreactive specificities, it has been proposed that stringent selection mechanisms prevent the development of autoreactive B cells. However, conventional assays to identify autoreactive B cells usually employ in vitro-generated Abs, which differ from membrane-bound BCRs. In this study, we used a cell-based assay to investigate the autoreactivity of membrane-bound BCRs derived from different B cell developmental stages of human peripheral blood. Contrasted to soluble Ab counterparts, only a few of the tested BCRs were autoreactive, although the cell-based assay sensitively detects feeble Ag recognition of a germline-reverted murine BCR that was selected after OVA immunization of mice, whereas conventional assays failed to do so. Together, these data suggest that proper identification of autoreactive B cells requires the membrane-bound BCR, as the soluble Ab may largely differ from its BCR counterpart in Ag binding.