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Status: Bibliographieeintrag

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Verfasst von:Sachdeva, Robin [VerfasserIn]   i
 Fleming, Thomas [VerfasserIn]   i
 Schumacher, Dagmar [VerfasserIn]   i
 Mohr, Franziska [VerfasserIn]   i
 Wagner, Andreas H. [VerfasserIn]   i
 Tsvilovskyy, Volodymyr [VerfasserIn]   i
 Mathar, Ilka [VerfasserIn]   i
 Freichel, Marc [VerfasserIn]   i
 Homberg, Sarah [VerfasserIn]   i
 Stilz, Kathrin [VerfasserIn]   i
 Mohr, Franziska [VerfasserIn]   i
Titel:Methylglyoxal evokes acute Ca2+ transients in distinct cell types and increases agonist-evoked Ca2+ entry in endothelial cells via CRAC channels
Verf.angabe:Robin Sachdeva, Thomas Fleming, Dagmar Schumacher, Sarah Homberg, Kathrin Stilz, Franziska Mohr, Andreas H. Wagner, Volodymyr Tsvilovskyy, Ilka Mathar, Marc Freichel
E-Jahr:2019
Jahr:09 January 2019
Umfang:10 S.
Fussnoten:Gesehen am 18.10.2019
Titel Quelle:Enthalten in: Cell calcium
Ort Quelle:Edinburgh [u.a.] : Churchill Livingstone, 1980
Jahr Quelle:2019
Band/Heft Quelle:78(2019), Seite 66-75
ISSN Quelle:1532-1991
Abstract:Methylglyoxal (MG) is a by-product of glucose metabolism and its accumulation has been linked to the development of diabetic complications such as retinopathy and nephropathy by affecting multiple signalling pathways. However, its influence on the intracellular Ca2+ homeostasis and particularly Ca2+ entry, which has been reported to be mediated via TRPA1 channels in DRG neurons, has not been studied in much detail in other cell types. In this study, we report the consequences of acute and long-term MG application on intracellular Ca2+ levels in endothelial cells. We showed that acute MG application doesn’t evoke any instantaneous changes in the intracellular Ca2+ concentration in immortalized mouse cardiac endothelial cells (MCECs) and murine microvascular endothelial cells (muMECs). In contrast, an MG-induced rise in intracellular Ca2+ level was observed in primary mouse mesangial cells within 30 s, indicating that the modulation of Ca2+ homeostasis by MG is strictly cell type specific. The formation of the MG-derived advanced glycation end product (AGE) MG-H1 was found to be time and concentration-dependent in MCECs. Likewise, MG pre-incubation for 6 h increased the angiotensin II-evoked Ca2+ entry in MCECs and muMECs which was abrogated by inhibition of Calcium release activated calcium (CRAC) channels with GSK-7975A, but unaffected by an inhibitor specific to TRPA1 channels. Quantitative PCR analysis revealed that MG pre-treatment did not affect expression of the genes encoding the angiotensin receptors AT1R (Agtr 1a & Agtr 1b), Trpa1 nor Orai1, Orai2, Orai3, Stim1, Stim2 and Saraf which operate as constituents or regulators of CRAC channels and store-operated Ca2+ entry (SOCE) in other cell types. Together, our results show that long-term MG stimulation leads to the formation of glycation end products, which facilitates the agonist-evoked Ca2+ entry in endothelial cells, and this could be a new pathway that might lead to MG-evoked vasoregression observed in diabetic vasculopathies.
DOI:doi:10.1016/j.ceca.2019.01.002
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.ceca.2019.01.002
 Volltext: http://www.sciencedirect.com/science/article/pii/S0143416018301787
 DOI: https://doi.org/10.1016/j.ceca.2019.01.002
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Calcium entry
 Calcium release activated calcium (CRAC) channels
 Endothelial cells
 Methylglyoxal
K10plus-PPN:1677069058
Verknüpfungen:→ Zeitschrift

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