| |  Online-Ressource |
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| Verfasst von: | Fuchs, Tina [VerfasserIn]  |
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| | Ries, Lukas [VerfasserIn] |
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| | Giesler, Sophie [VerfasserIn] |
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| | Busch, Svenja [VerfasserIn] |
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| | Kaminski, Wolfgang E. [VerfasserIn] |
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| | Neumaier, Michael [VerfasserIn] |
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| Titel: | Expression of combinatorial immunoglobulins in macrophages in the tumor microenvironment |
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| Verf.angabe: | Tina Fuchs, Martin Hahn, Lukas Ries, Sophie Giesler, Svenja Busch, Chunlin Wang, Jian Han, Torsten J. Schulze, Kerstin Puellmann, Alexander W. Beham, Wolfgang E. Kaminski, Michael Neumaier |
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| E-Jahr: | 2018 |
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| Jahr: | September 21, 2018 |
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| Umfang: | 19 S. |
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| Fussnoten: | Gesehen am 16.09.2019 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 13(2018,9) Artikel-Nummer e0204108, 19 Seiten |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | Recent evidence indicates the presence of macrophage subpopulations that express the TCRαβ in chronic inflammatory diseases such as tuberculosis and atherosclerosis and in the tumor microenvironment. Here, we demonstrate that a second subpopulation of macrophages expresses rearranged heavy and light chain immunoglobulins. We identify immunoglobulin expression in human and murine monocytes, in ex vivo differentiated macrophages and macrophages from the tumor microenvironment of five randomly selected distinct human tumor entities. The immunoglobulin heavy and light chains are expressed in a small macrophage subfraction (~3-5%) as combinatorial and individual-specific immune receptors. Using Sanger sequencing and deep sequencing, we routinely find markedly restricted Ig repertoires in monocytes/macrophages compared to normal B cells. Furthermore, we report the complete Ig heavy and light chain sequences of a fully functional immunoglobulin from a single tumor-associated macrophage. These results demonstrate that Ig expression is a defining feature of monocytes and also macrophages in the tumor microenvironment and thus reveal an as yet unrecognized modus operandi of host defense in professional phagocytes. |
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| DOI: | doi:10.1371/journal.pone.0204108 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1371/journal.pone.0204108 |
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| | kostenfrei: Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204108 |
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| | DOI: https://doi.org/10.1371/journal.pone.0204108 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Antibodies |
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| | B cells |
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| | Macrophages |
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| | Monocytes |
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| | Mouse models |
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| | Polymerase chain reaction |
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| | Reverse transcriptase-polymerase chain reaction |
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| | Sequence databases |
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| K10plus-PPN: | 1677122404 |
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| Verknüpfungen: | → Zeitschrift |
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Expression of combinatorial immunoglobulins in macrophages in the tumor microenvironment / Fuchs, Tina [VerfasserIn]; September 21, 2018 (Online-Ressource)
