Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Even, Ipek [VerfasserIn]   i
 Reidenbach, Sonja [VerfasserIn]   i
 Hofmann, Ilse [VerfasserIn]   i
Titel:DLIC1, but not DLIC2, is upregulated in colon cancer and this contributes to proliferative overgrowth and migratory characteristics of cancer cells
Verf.angabe:Ipek Even, Sonja Reidenbach, Tanja Schlechter, Nicola Berns, Rosanna Herold, Wilfried Roth, Damir Krunic, Veit Riechmann and Ilse Hofmann
Jahr:2019
Umfang:18 S.
Fussnoten:Gesehen am 18.09.2019
Titel Quelle:Enthalten in: Vereinigung der Europäischen Biochemischen GesellschaftenThe FEBS journal
Ort Quelle:Oxford [u.a.] : Wiley-Blackwell, 2005
Jahr Quelle:2019
Band/Heft Quelle:286(2019), 4, Seite 803-820
ISSN Quelle:1742-4658
Abstract:Cytoplasmic dynein-1 is a large minus-end-directed microtubule motor complex involved in membrane trafficking, organelle positioning, and microtubule organization. The roles of dynein light intermediate chains (DLICs; DLIC1 and DLIC2) within the complex are, however, still largely undefined. In this study, we investigated the possible roles of DLICs in epithelial homeostasis and colon cancer development. Mutant clonal analysis of Drosophila Dlic in the follicular epithelium of Drosophila ovary showed defects in nuclear positioning, epithelial integrity, and apical cell polarity. Consistently, knockdown of human DLIC1 and DLIC2 in colon carcinoma cells resulted in damaged epithelial organization, disturbed lumen formation, and impaired apical polarity establishment in three-dimensional cell culture. Depletion of DLIC1 and DLIC2 led to reduced proliferation, enhanced apoptosis rates, disrupted mitotic spindle assembly, and induction of G2/M arrest in cell cycle progression. Moreover, reduced levels of DLIC1 in contrast to DLIC2 impaired the migratory ability. On the other hand, immunohistochemical examination of human colorectal tissue samples and further colorectal cancer dataset analysis showed a significant upregulation for DLIC1 in tumors, whereas DLIC2 expression was unchanged. In addition, the overexpression of DLIC1 caused increased proliferation, decreased apoptosis and enhanced migration, whereas DLIC2 overexpression did not result in any significant changes. Together, these results indicate that DLIC1 and DLIC2 contribute to the establishment and maintenance of epithelial homeostasis. Furthermore, these findings present the first evidence that DLIC1 and DLIC2 have distinct roles in colon cancer development and that DLIC1 may contribute to proliferative overgrowth and migratory characteristics.
DOI:doi:10.1111/febs.14755
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1111/febs.14755
 Volltext: https://febs.onlinelibrary.wiley.com/doi/abs/10.1111/febs.14755
 DOI: https://doi.org/10.1111/febs.14755
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:colon cancer
 cytoplasmic dynein 1
 dynein light intermediate chain
 epithelial homeostasis
K10plus-PPN:1677241705
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68431101   QR-Code
zum Seitenanfang