Kocuria tytonis sp. nov., isolated from the uropygial gland of an American barn owl (Tyto furcata)

• Verfasst von: Braun, Markus S. [VerfasserIn]
• Verfasst von: Wang, Erjia [VerfasserIn]
• Verfasst von: Zimmermann, Stefan [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Kocuria tytonis sp. nov., isolated from the uropygial gland of an American barn owl (Tyto furcata)
• Verf.angabe: Markus Santhosh Braun, Erjia Wang, Stefan Zimmermann, Hermann Wagner, Michael Wink
• E-Jahr: 2019
• Jahr: 10 January 2019
• Umfang: 5 S.
• Fussnoten: Gesehen am 19.11.2019
• Titel Quelle: Enthalten in: International journal of systematic and evolutionary microbiology
• Ort Quelle: Reading : SGM, 1951
• Jahr Quelle: 2019
• Band/Heft Quelle: 69(2019), 2, Seite 447-451
• ISSN Quelle: 1466-5034
• ISSN Quelle: 1465-2102
• DOI: doi:10.1099/ijsem.0.003170
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1678026557

Abstract

Avian uropygial glands have received increasing attention in recent years, but little is known about micro-organisms in uropygial glands. In this study, we isolated a strain of Gram-stain-positive, non-motile, non-spore-forming cocci, designated 442<span class="jp-sup">T</span>, from the uropygial gland of an American barn owl (<em class="">Tyto furcata</em>) and characterized it using a polyphasic approach. 16S rRNA gene sequence analysis placed the isolate in the genus <em class=""> <span class="named-content-genus"> <a class="namesforlife" href="https://doi.org/10.1601/nm.5886" rel="namesforlife-name" target="xrefwindow" title="Kocuria">Kocuria</a> </span></em>. The G+C content was 70.8 mol%, the major menaquinone was MK-7(H<sub class="">2</sub>) and the predominant cellular fatty acids were anteiso-C<sub class="">15:0</sub>, anteiso-C<sub class="">17:0</sub> and iso-C<sub class="">15:0</sub>. Phylogenetic analyses based on the 16S rRNA gene identified <em class=""> <span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.5892" rel="namesforlife-name" target="xrefwindow" title="Kocuria rhizophila">Kocuria rhizophila</a></span></em> DSM 11926<span class="jp-sup">T</span> (99.6% similarity), <em class=""><span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.21139" rel="namesforlife-name" target="xrefwindow" title="Kocuria salsicia">Kocuria salsicia</a></span></em> DSM 24776<span class="jp-sup">T</span> (98.7%), <em class=""> <span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.5893" rel="namesforlife-name" target="xrefwindow" title="Kocuria varians">Kocuria varians</a></span></em> DSM 20033<span class="jp-sup">T</span> (98.3%) and <em class=""><span class="named-content-species"> <a class="namesforlife" href="https://doi.org/10.1601/nm.8647" rel="namesforlife-name" target="xrefwindow" title="Kocuria marina">Kocuria marina</a> </span> </em> DSM 16420<span class="jp-sup">T</span> (98.3%) as the most closely related species. However, average nucleotide identity values below 86% indicated that the isolate differed from all species hitherto described. Chemotaxonomic analyses and whole-cell protein profiles corroborated these findings. Accordingly, the isolate is considered to be a member of a novel species, for which the name <em class=""><span class="named-content-genus"><a class="namesforlife" href="https://doi.org/10.1601/nm.5886" rel="namesforlife-name" target="xrefwindow" title="Kocuria">Kocuria</a></span> tytonis</em> sp. nov. is proposed. The type strain is 442<span class="jp-sup">T</span> (=DSM 104130<span class="jp-sup">T</span>=LMG 29944<span class="jp-sup">T</span>).,