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| Verfasst von: | Balta, Emre [VerfasserIn]  |
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| | Hardt, Robert [VerfasserIn] |
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| | Liang, Jie [VerfasserIn] |
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| | Orlik, Christian [VerfasserIn] |
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| | Hillmer, Stefan [VerfasserIn] |
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| | Meuer, Stefan [VerfasserIn] |
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| | Hübner, Katrin [VerfasserIn] |
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| | Wabnitz, Guido H. [VerfasserIn] |
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| | Samstag, Yvonne [VerfasserIn] |
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| Titel: | Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells |
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| Verf.angabe: | Emre Balta, Robert Hardt, Jie Liang, Henning Kirchgessner, Christian Orlik, Beate Jahraus, Stefan Hillmer, Stefan Meuer, Katrin Hübner, Guido H. Wabnitz & Yvonne Samstag |
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| E-Jahr: | 2019 |
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| Jahr: | 09 September 2019 |
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| Umfang: | 18 S. |
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| Fussnoten: | Gesehen am 07.10.2019 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 10(2019) Artikel-Nummer 4073, 18 Seiten |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Several antitumor therapies work by increasing reactive oxygen species (ROS) within the tumor micromilieu. Here, we reveal that L-plastin (LPL), an established tumor marker, is reversibly regulated by ROS-induced thiol oxidation on Cys101, which forms a disulfide bridge with Cys42. LPL reduction is mediated by the Thioredoxin1 (TRX1) system, as shown by TRX1 trapping, TRX1 knockdown and blockade of Thioredoxin1 reductase (TRXR1) with auranofin. LPL oxidation diminishes its actin-bundling capacity. Ratiometric imaging using an LPL-roGFP-Orp1 fusion protein and a dimedone-based proximity ligation assay (PLA) reveal that LPL oxidation occurs primarily in actin-based cellular extrusions and strongly inhibits cell spreading and filopodial extension formation in tumor cells. This effect is accompanied by decreased tumor cell migration, invasion and extracellular matrix (ECM) degradation. Since LPL oxidation occurs following treatment of tumors with auranofin or γ-irradiation, it may be a molecular mechanism contributing to the effectiveness of tumor treatment with redox-altering therapies. |
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| DOI: | doi:10.1038/s41467-019-11909-z |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41467-019-11909-z |
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| | Verlag: https://www.nature.com/articles/s41467-019-11909-z |
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| | DOI: https://doi.org/10.1038/s41467-019-11909-z |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1678194921 |
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| Verknüpfungen: | → Zeitschrift |
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Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells / Balta, Emre [VerfasserIn]; 09 September 2019 (Online-Ressource)
