Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions

• Verfasst von: Jaiswal, Astha [VerfasserIn]
• Verfasst von: Lorenz, Holger [VerfasserIn]
• Titel: Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions
• Verf.angabe: Astha Jaiswal, Christian H. Hoerth, Ana M. Zúñiga Pereira & Holger Lorenz
• E-Jahr: 2019
• Jahr: 09 September 2019
• Umfang: 13 S.
• Fussnoten: Gesehen am 07.10.2019
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2019
• Band/Heft Quelle: 9(2019) Artikel-Nummer 12911, 13 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/s41598-019-49408-2
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1678196347

Abstract

Induced morphology changes of cells and organelles are by far the easiest way to determine precise protein sub-locations and organelle quantities in light microscopy. By using hypotonic solutions to swell mammalian cell organelles we demonstrate that precise membrane, lumen or matrix protein locations within the endoplasmic reticulum, Golgi and mitochondria can reliably be established. We also show the benefit of this approach for organelle quantifications, especially for clumped or intertwined organelles like peroxisomes and mitochondria. Since cell and organelle swelling is reversible, it can be applied to live cells for successive high-resolution analyses. Our approach outperforms many existing imaging modalities with respect to resolution, ease-of-use and cost-effectiveness without excluding any co-utilization with existing optical (super)resolution techniques.