Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration

• Verfasst von: Lucic, Bojana [VerfasserIn]
• Verfasst von: Wei, Wang [VerfasserIn]
• Verfasst von: Schmidt, Manfred [VerfasserIn]
• Verfasst von: Lusic, Marina [VerfasserIn]
• Titel: Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration
• Verf.angabe: Bojana Lucic, Heng-Chang Chen, Maja Kuzman, Eduard Zorita, Julia Wegner, Vera Minneker, Wei Wang, Raffaele Fronza, Stefanie Laufs, Manfred Schmidt, Ralph Stadhouders, Vassilis Roukos, Kristian Vlahovicek, Guillaume J. Filion & Marina Lusic
• E-Jahr: 2019
• Jahr: 06 September 2019
• Umfang: 12 S.
• Fussnoten: Gesehen am 07.10.2019
• Titel Quelle: Enthalten in: Nature Communications
• Ort Quelle: [London] : Nature Publishing Group UK, 2010
• Jahr Quelle: 2019
• Band/Heft Quelle: 10(2019) Artikel-Nummer 4059, 12 Seiten
• ISSN Quelle: 2041-1723
• DOI: doi:10.1038/s41467-019-12046-3
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1678214418

Abstract

HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently targeted genes are proximal to super-enhancer genomic elements and that they cluster in specific spatial compartments of the T cell nucleus. We further show that these gene clusters acquire their location during the activation of T cells. The clustering of these genes along with their transcriptional activity are the major determinants of HIV-1 integration in T cells. Our results provide evidence of the relevance of the spatial compartmentalization of the genome for HIV-1 integration, thus further strengthening the role of nuclear architecture in viral infection.