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| Verfasst von: | Hata, Shoji [VerfasserIn]  |
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| | Pastor Peidro, Ana [VerfasserIn] |
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| | Panić, Marko [VerfasserIn] |
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| | Liu, Peng [VerfasserIn] |
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| | Atorino, Enrico [VerfasserIn] |
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| | Jäkle, Ursula [VerfasserIn] |
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| | Pereira, Gislene [VerfasserIn] |
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| | Schiebel, Elmar [VerfasserIn] |
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| Titel: | The balance between KIFC3 and EG5 tetrameric kinesins controls the onset of mitotic spindle assembly |
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| Verf.angabe: | Shoji Hata, Ana Pastor Peidro, Marko Panic, Peng Liu, Enrico Atorino, Charlotta Funaya, Ursula Jäkle, Gislene Pereira and Elmar Schiebel |
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| E-Jahr: | 2019 |
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| Jahr: | 02 September 2019 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 09.10.2019 |
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| Titel Quelle: | Enthalten in: Nature cell biology |
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| Ort Quelle: | New York, NY : Nature America, 1999 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 21(2019), 9, Seite 1138-1151 |
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| ISSN Quelle: | 1476-4679 |
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| Abstract: | One of the first steps in mitotic spindle assembly is the dissolution of the centrosome linker followed by centrosome separation driven by EG5, a tetrameric plus-end-directed member of the kinesin-5 family. However, even in the absence of the centrosome linker, the two centrosomes are kept together by an ill-defined microtubule-dependent mechanism. Here we show that KIFC3, a minus-end-directed kinesin-14, provides microtubule-based centrosome cohesion. KIFC3 forms a homotetramer that pulls the two centrosomes together via a specific microtubule network. At mitotic onset, KIFC3 activity becomes the main driving force of centrosome cohesion to prevent premature spindle formation after linker dissolution as it counteracts the increasing EG5-driven pushing forces. KIFC3 is eventually inactivated by NEver in mitosis-related Kinase 2 (NEK2) to enable EG5-driven bipolar spindle assembly. We further show that persistent centrosome cohesion in mitosis leads to chromosome mis-segregation. Our findings reveal a mechanism of spindle assembly that is evolutionary conserved from yeast to humans. |
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| DOI: | doi:10.1038/s41556-019-0382-6 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41556-019-0382-6 |
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| | Verlag: https://www.nature.com/articles/s41556-019-0382-6 |
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| | DOI: https://doi.org/10.1038/s41556-019-0382-6 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1678545619 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ balance between KIFC3 and EG5 tetrameric kinesins controls the onset of mitotic spindle assembly / Hata, Shoji [VerfasserIn]; 02 September 2019 (Online-Ressource)
