Styphnolobium japonicum (L.) Schott fruits increase stress resistance and exert antioxidant properties in Caenorhabditis elegans and mouse models

• Verfasst von: Thabit, Sara [VerfasserIn]
• Verfasst von: Handoussa, Heba [VerfasserIn]
• Verfasst von: Roxo, Mariana [VerfasserIn]
• Verfasst von: Cestari de Azevedo, Bruna [VerfasserIn]
• Verfasst von: S. E. El Sayed, Nesrine [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Styphnolobium japonicum (L.) Schott fruits increase stress resistance and exert antioxidant properties in Caenorhabditis elegans and mouse models
• Verf.angabe: Sara Thabit, Heba Handoussa, Mariana Roxo, Bruna Cestari de Azevedo, Nesrine S. E. El Sayed and Michael Wink
• E-Jahr: 2019
• Jahr: 19 July 2019
• Umfang: 20 S.
• Fussnoten: Gesehen am 17.10.2019
• Titel Quelle: Enthalten in: Molecules
• Ort Quelle: Basel : MDPI, 1996
• Jahr Quelle: 2019
• Band/Heft Quelle: 24(2019,14) Artikel-Nummer 2633, 20 Seiten
• ISSN Quelle: 1420-3049
• DOI: doi:10.3390/molecules24142633
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: <i>Caenorhabditis elegans</i>
• Sach-SW: <i>Styphnolobium japonicum</i>
• Sach-SW: antioxidant
• Sach-SW: HPLC-PDA-ESI-MS/MS
• Sach-SW: oxidative stress
• K10plus-PPN: 1679085972
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Styphnolobium japonicum (L.) Schott is a popular Asian tree widely used in traditional medicine. The current study explored the potential stress resistance and antioxidant activities of its fruits. Phytochemical profiling of the hydroalcoholic fruit extract was done via high performance liquid chromatography-photodiode array-electrospray ionization-mass/mass (HPLC-PDA-ESI-MS/MS). Twenty four phenolic constituents were tentatively identified in the extract. The Caenorhabditis elegans (C. elegans) nematode model in addition to trimethyltin (TMT)-induced neurotoxicity mouse model were used for in vivo evaluation of its antioxidant properties. The ability of the extract to enhance stress resistance was manifested through increasing survival rate by 44.7% and decreasing basal reactive oxygen species (ROS) levels by 72.3% in C. elegans. In addition, the extract increased the levels of the stress response enzyme superoxide dismutase-3 (Sod-3) by 55.5% and decreased the expression of heat shock protein-16.2 (Hsp-16.2) in nematodes, which had been challenged by juglone, by 21%. Using a mouse model, the extract significantly decreased the expression of the oxidative stress marker malondialdehyde (MDA). Furthermore, an elevation in the levels of the antioxidant marker glutathione (GSH), SOD and heme oxygenase-1 (HO-1) enzymes were observed. Our findings imply that Styphnolobium japonicum has the potential to be used in future studies focusing on diseases associated with oxidative stress.