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Verfasst von:Krebs, Jörg [VerfasserIn]   i
 Hillenbrand, Alexander [VerfasserIn]   i
 Tsagogiorgas, Charalambos [VerfasserIn]   i
 Patry, Christian [VerfasserIn]   i
 Tönshoff, Burkhard [VerfasserIn]   i
 Yard, Benito A. [VerfasserIn]   i
 Beck, Grietje [VerfasserIn]   i
 Rafat, Neysan [VerfasserIn]   i
Titel:Intravenous delivery of granulocyte-macrophage colony stimulating factor impairs survival in lipopolysaccharide-induced sepsis
Verf.angabe:Jörg Krebs, Alexander Hillenbrand, Charalambos Tsagogiorgas, Christian Patry, Burkhard Tönshoff, Benito Yard, Grietje Beck, Neysan Rafat
E-Jahr:2019
Jahr:June 20, 2019
Umfang:14 S.
Fussnoten:Gesehen am 17.10.2019
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2019
Band/Heft Quelle:14(2019,6) Artikel-Nummer e0218602, 14 Seiten
ISSN Quelle:1932-6203
Abstract:Background Cell-based therapies with bone marrow-derived progenitor cells (BMDPC) lead to an improved clinical outcome in animal sepsis models. In the present study we evaluated the ability of granulocyte macrophage-colony stimulating factor (GM-CSF) to mobilize BMDPC in a lipopolysaccharide (LPS)-induced sepsis model and thereby its potential as a novel treatment strategy. Methods Male Wistar rats received LPS (25μg/kg/h for 4 days) intravenously and were subsequently treated with GM-CSF 12.5μg/kg (0h,24h,48h,72h). As control groups, rats were infused with sodium chloride or GM-CSF only. Clinical and laboratory parameters, proinflammatory plasma cytokines as well as BMDPC counts were analyzed. Cytokine release by isolated peripheral blood mononuclear cells from rat spleen upon incubation with LPS, GM-CSF and a combination of both were investigated in vitro. Results In vivo, rats receiving both LPS and GM-CSF, showed a reduced weight loss and increased mobilization of BMDPC. At the same time, this regime resulted in an increased release of proinflammatory cytokines (IL-6, IL-8) and a significantly increased mortality. In vitro, the combination of LPS and GM-CSF showed a significantly increased IL-6 release upon incubation compared to incubation with LPS or GM-CSF alone. Conclusions GM-CSF did not have a beneficial effect on the clinical course in our LPS-induced sepsis model. It synergistically promoted inflammation with LPS and probably thereby impaired survival.
DOI:doi:10.1371/journal.pone.0218602
URL:kostenfrei: Volltext: https://doi.org/10.1371/journal.pone.0218602
 kostenfrei: Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0218602
 DOI: https://doi.org/10.1371/journal.pone.0218602
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Blood
 Cytokines
 Death rates
 Endotoxins
 Enzyme-linked immunoassays
 Sepsis
 Spleen
 Stem cells
K10plus-PPN:1679088351
Verknüpfungen:→ Zeitschrift
 
 
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