| |  Online-Ressource |
|---|
| Verfasst von: | Uddin, Borhan [VerfasserIn]  |
|---|
| | Chen, Nan-Peng [VerfasserIn] |
|---|
| | Neuner, Annett [VerfasserIn] |
|---|
| | Ruppert, Thomas [VerfasserIn] |
|---|
| | Lorenz, Holger [VerfasserIn] |
|---|
| | Pereira, Gislene [VerfasserIn] |
|---|
| | Schiebel, Elmar [VerfasserIn] |
|---|
| | Partscht, Patrick [VerfasserIn] |
|---|
| Titel: | The human phosphatase CDC14A modulates primary cilium length by regulating centrosomal actin nucleation |
|---|
| Verf.angabe: | Borhan Uddin, Patrick Partscht, Nan-Peng Chen, Annett Neuner, Manuel Weiß, Robert Hardt, Aliakbar Jafarpour, Bernd Heßling, Thomas Ruppert, Holger Lorenz, Gislene Pereira & Elmar Schiebel |
|---|
| Jahr: | 2019 |
|---|
| Fussnoten: | Published online 22 November 2018 ; Gesehen am 21.10.2019 |
|---|
| Titel Quelle: | Enthalten in: European Molecular Biology OrganizationEMBO reports |
|---|
| Ort Quelle: | Heidelberg : EMBO Press, 2000 |
|---|
| Jahr Quelle: | 2019 |
|---|
| Band/Heft Quelle: | 20(2019,1) Artikel-Nummer e46544, 16 Seiten |
|---|
| ISSN Quelle: | 1469-3178 |
|---|
| Abstract: | Abstract CDC14A codes for a conserved proline-directed phosphatase, and mutations in the gene are associated with autosomal-recessive severe to profound deafness, due to defective kinocilia. A role of CDC14A in cilia formation has also been described in other organisms. However, how human CDC14A impacts on cilia formation remains unclear. Here, we show that human RPE1 hCDC14APD cells, encoding a phosphatase dead version of hCDC14A, have longer cilia than wild-type cells, while hCDC14A overexpression reduces cilia formation. Phospho-proteome analysis of ciliated RPE1 cells identified actin-associated and microtubule binding proteins regulating cilia length as hCDC14A substrates, including the actin-binding protein drebrin. Indeed, we find that hCDC14A counteracts the CDK5-dependent phosphorylation of drebrin at S142 during ciliogenesis. Further, we show that drebrin and hCDC14A regulate the recruitment of the actin organizer Arp2 to centrosomes. In addition, during ciliogenesis hCDC14A also regulates endocytosis and targeting of myosin Va vesicles to the basal body in a drebrin-independent manner, indicating that it impacts primary cilia formation in a multilayered manner. |
|---|
| DOI: | doi:10.15252/embr.201846544 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.15252/embr.201846544 |
|---|
| | Verlag: https://www.embopress.org/doi/full/10.15252/embr.201846544 |
|---|
| | DOI: https://doi.org/10.15252/embr.201846544 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | actin |
|---|
| | CDK5 |
|---|
| | DBN1 |
|---|
| | hCDC14A |
|---|
| | primary cilium |
|---|
| K10plus-PPN: | 1679159933 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
¬The¬ human phosphatase CDC14A modulates primary cilium length by regulating centrosomal actin nucleation / Uddin, Borhan [VerfasserIn]; 2019 (Online-Ressource)
