Rheum palmatum root extract inhibits hepatocellular carcinoma in rats treated with diethylnitrosamine

• Verfasst von: El‐Saied, Mahmoud A. [VerfasserIn]
• Verfasst von: Sobeh, Mansour [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Rheum palmatum root extract inhibits hepatocellular carcinoma in rats treated with diethylnitrosamine
• Verf.angabe: Mahmoud A. El‐Saied, Mansour Sobeh, Walied Abdo, Osama M. Badr, Lamiaa T. Youssif, Ibrahim H. Elsayed, Samir M. Osman, Michael Wink
• E-Jahr: 2018
• Jahr: 09 March 2018
• Umfang: 9 S.
• Fussnoten: Gesehen am 21.10.2019
• Titel Quelle: Enthalten in: Journal of pharmacy and pharmacology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1949
• Jahr Quelle: 2018
• Band/Heft Quelle: 70(2018), 6, Seite 821-829
• ISSN Quelle: 2042-7158
• DOI: doi:10.1111/jphp.12899
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: diethylnitrosamine
• Sach-SW: DNA fragmentation
• Sach-SW: hepatocellular carcinoma
• Sach-SW: HPLC-MS/MS
• Sach-SW: Rheum palmatum
• K10plus-PPN: 1679167227

Abstract

Objectives The aim of this study was to investigate the potential anticancer properties of a methanol extract of Rheum palmatum roots against diethylnitrosamine (DENA)-induced hepatocellular carcinoma (HCC) in rats and to characterize its phytoconstituents. Methods HPLC-PDA-MS/MS was used to profile the secondary metabolites in R. palmatum root extract. HCC was induced using diethylnitrosamine (DENA). The activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT), alpha-fetoprotein (AFP), total proteins, serum albumin and serum globulin was determined. DNA fragmentation and histopathological examination and GST-P immunostaining were also studied. Key findings LC-MS/MS analysis identified 16 compounds belonging to anthraquinones, flavonoids and tannins. The root extract significantly reduced the elevated liver enzymes ALT and AST and increased total proteins, albumin and globulin in HCC-rats. Also, the tumour markers AFP and GGT levels were significantly reduced in HCC-rats treated with the extract. In addition, the extract significantly reduced elevated DNA fragmentation and decreased the numbers and areas of GST-P positive putative foci in HCC-rats. Conclusions Rheum palmatum is a potential candidate to be explored for the treatment of hepatocellular carcinoma.