Status: Bibliographieeintrag
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| Verfasst von: | Salomon, Johanna J. [VerfasserIn]  |
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| Titel: | OCTN2-mediated acetyl-l-carnitine transport in human pulmonary epithelial cells in vitro |
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| Verf.angabe: | Johanna J. Salomon, Julia C. Gausterer, Mohammed Ali Selo, Ken-ichi Hosoya, Hanno Huwer, Nicole Schneider-Daum, Claus-Michael Lehr and Carsten Ehrhardt |
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| E-Jahr: | 2019 |
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| Jahr: | 7 August 2019 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 21.10.2019 |
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| Titel Quelle: | Enthalten in: Pharmaceutics |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 11(2019,8) Artikel-Nummer 396, 11 Seiten |
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| ISSN Quelle: | 1999-4923 |
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| Abstract: | The carnitine transporter OCTN2 is associated with asthma and other inflammatory diseases. The aims of this work were (i) to determine carnitine uptake into freshly isolated human alveolar type I (ATI)-like epithelial cells in primary culture, (ii) to compare the kinetics of carnitine uptake between respiratory epithelial in vitro cell models, and (iii) to establish whether any cell line was a suitable model for studies of carnitine transport at the air-blood barrier. Levels of time-dependent [3H]-acetyl-l-carnitine uptake were similar in ATI-like, NCl-H441, and Calu-3 epithelial cells, whereas uptake into A549 cells was ~5 times higher. Uptake inhibition was more pronounced by OCTN2 modulators, such as l-Carnitine and verapamil, in ATI-like primary epithelial cells compared to NCl-H441 and Calu-3 epithelial cells. Our findings suggest that OCTN2 is involved in the cellular uptake of acetyl-l-carnitine at the alveolar epithelium and that none of the tested cell lines are optimal surrogates for primary cells. |
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| DOI: | doi:10.3390/pharmaceutics11080396 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/pharmaceutics11080396 |
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| | Verlag: https://www.mdpi.com/1999-4923/11/8/396 |
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| | DOI: https://doi.org/10.3390/pharmaceutics11080396 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | acetyl-<span style="font-variant: small-caps">l</span>-carnitine |
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| | asthma |
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| | epithelial transport |
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| | in vitro models |
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| | lung epithelium |
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| | OCTN2 |
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| | organic cation transporter |
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| K10plus-PPN: | 167917066X |
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| Verknüpfungen: | → Zeitschrift |
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OCTN2-mediated acetyl-l-carnitine transport in human pulmonary epithelial cells in vitro / Salomon, Johanna J. [VerfasserIn]; 7 August 2019 (Online-Ressource)
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