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Verfasst von:Kriegsmann, Jörg Bert [VerfasserIn]   i
 Kriegsmann, Mark [VerfasserIn]   i
 Kriegsmann, Katharina [VerfasserIn]   i
 Longuespée, Rémi [VerfasserIn]   i
Titel:MALDI imaging for proteomic painting of heterogeneous tissue structures
Verf.angabe:Jörg Kriegsmann, Mark Kriegsmann, Katharina Kriegsmann, Rémi Longuespée, Sören-Oliver Deininger, and Rita Casadonte
Jahr:2019
Jahr des Originals:2018
Fussnoten:First published: 24 November 2018 ; Gesehen am 23.10.2019
Titel Quelle:Enthalten in: Proteomics / Clinical applications
Ort Quelle:Weinheim : Wiley VCH, 2007
Jahr Quelle:2019
Band/Heft Quelle:13(2019,1) Artikel-Nummer 1800045, 10 Seiten
ISSN Quelle:1862-8354
Abstract:Purpose To present matrix-assisted laser desorption/ionization (MALDI) imaging as a powerful method to highlight various tissue compartments. Experimental design Formalin-fixed paraffin-embedded (FFPE) tissue of a uterine cervix, a pancreas, a duodenum, a teratoma, and a breast cancer tissue microarray (TMA) are analyzed by MALDI imaging and by immunohistochemistry (IHC). Peptide images are visualized and analyzed using FlexImaging and SCiLS Lab software. Different histological compartments are compared by hierarchical cluster analysis. Results MALDI imaging highlights tissue compartments comparable to IHC. In cervical tissue, normal epithelium can be discerned from intraepithelial neoplasia. In pancreatic and duodenal tissues, m/z signals from lymph follicles, vessels, duodenal mucosa, normal pancreas, and smooth muscle structures can be visualized. In teratoma, specific m/z signals to discriminate squamous epithelium, sebaceous glands, and soft tissue are detected. Additionally, tumor tissue can be discerned from the surrounding stroma in small tissue cores of TMAs. Proteomic data acquisition of complex tissue compartments in FFPE tissue requires less than 1 h with recent mass spectrometers. Conclusion and clinical relevance The simultaneous characterization of morphological and proteomic features in the same tissue section adds proteomic information for histopathological diagnostics, which relies at present on conventional hematoxylin and eosin staining, histochemical, IHC and molecular methods.
DOI:doi:10.1002/prca.201800045
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1002/prca.201800045
 Verlag: https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800045
 DOI: https://doi.org/10.1002/prca.201800045
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:formalin-fixed paraffin-embedded
 immunohistochemistry
 MALDI imaging
 tumor heterogeneity
K10plus-PPN:1679464671
Verknüpfungen:→ Zeitschrift

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