Endothelial cell modulation of cardiomyocyte gene expression

• Verfasst von: Jiang, Fan [VerfasserIn]
• Verfasst von: Mohr, Franziska [VerfasserIn]
• Verfasst von: Ullrich, Nina D. [VerfasserIn]
• Verfasst von: Hecker, Markus [VerfasserIn]
• Verfasst von: Wagner, Andreas H. [VerfasserIn]
• Titel: Endothelial cell modulation of cardiomyocyte gene expression
• Verf.angabe: Fan Jiang, Franziska Mohr, Nina D. Ullrich, Markus Hecker, Andreas H. Wagner
• E-Jahr: 2019
• Jahr: 20 August 2019
• Umfang: 9 S.
• Fussnoten: Gesehen am 24.10.2019
• Titel Quelle: Enthalten in: Experimental cell research
• Ort Quelle: Orlando, Fla. : Academic Press, 1950
• Jahr Quelle: 2019
• Band/Heft Quelle: 383(2019) Artikel-Nummer 111565, 9 Seiten
• ISSN Quelle: 1090-2422
• DOI: doi:10.1016/j.yexcr.2019.111565
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Angiopoietin-2
• Sach-SW: Cardiomyocytes
• Sach-SW: Co-culture
• Sach-SW: Endothelial cells
• Sach-SW: Endothelin-1
• Sach-SW: Fetal gene expression
• K10plus-PPN: 1679545787

Abstract

The anatomic arrangement of microvascular endothelial cells and cardiomyocytes in vivo enables close interactions among these cells. In our in vitro co-culture system, ANP and BNP expression in the mouse atrial cardiomyocyte cell line HL-1 and subsequent ANP release were significantly upregulated when co-cultured with mouse cardiac microvascular endothelial cells or exposed to endothelial cell-conditioned medium. Endothelin-1 (ET-1) activation of endothelial cells remarkably enhanced their paracrine effect on cardiomyocyte gene expression, suggesting that ET-1 stimulation of endothelial cells affects expression of fetal genes such as ANP and BNP in adult cardiomyocytes through paracrine signalling. Exposure of HL-1cells and murine induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) to authentic angiopoietin-2 (Ang2) caused a concentration-dependent decrease in ANP expression while ET-1-induced ANP expression was augmented by low but inhibited by high concentrations of Ang2. FK506-mediated inhibition of the calcineurin-NFAT pathway in the HL-1cells selectively inhibited the stimulatory effect of the conditioned medium derived from ET-1-pre-stimulated endothelial cells on cardiomyocyte fetal gene expression. Combined with previous results indicating a crucial role for ANP and BNP in cardiac homeostasis, our findings provide further evidence that paracrine signalling by cardiac microvascular endothelial cells modulates cardiomyocyte function.