HPV DNA Associates with breast cancer malignancy and it is transferred to breast cancer stromal cells by extracellular vesicles

• Verfasst von: De Carolis, Sabrina [VerfasserIn]
• Verfasst von: Gallucci, Lara [VerfasserIn]
• Titel: HPV DNA Associates with breast cancer malignancy and it is transferred to breast cancer stromal cells by extracellular vesicles
• Verf.angabe: Sabrina De Carolis, Gianluca Storci, Claudio Ceccarelli, Claudia Savini, Lara Gallucci, Pasquale Sansone, Donatella Santini, Renato Seracchioli, Mario Taffurelli, Francesco Fabbri, Fabrizio Romani, Gaetano Compagnone, Cristina Giuliani, Paolo Garagnani, Massimiliano Bonafè and Monica Cricca
• E-Jahr: 2019
• Jahr: 16 September 2019
• Umfang: 12 S.
• Fussnoten: Gesehen am 25.10.2019
• Titel Quelle: Enthalten in: Frontiers in oncology
• Ort Quelle: Lausanne : Frontiers Media, 2011
• Jahr Quelle: 2019
• Band/Heft Quelle: 9(2019) Artikel-Nummer 860, 12 Seiten
• ISSN Quelle: 2234-943X
• DOI: doi:10.3389/fonc.2019.00860
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: circulating HPV DNA
• Sach-SW: Extracellular vesicles (EVs)
• Sach-SW: Human papillomavirus (HPV)
• Sach-SW: Stromal Cells
• Sach-SW: Triple negative BC
• K10plus-PPN: 1679999443

Abstract

A causal link between Human Papillomavirus (HPV) and breast cancer (BC) remains controversial. In spite of this, the observation that HPV DNA is over-represented in the Triple Negative (TN) BC has been reported. Here we remark the high prevalence of HPV DNA (44.4%) in aggressive BC subtypes (TN and HER2+) in a population of 273 Italian women and we convey the presence of HPV DNA in the epithelial and stromal compartments by in situ hybridization. As previously reported, we also found that serum derived-extracellular vesicles (EVs) from BC affected patients contain HPV DNA. Interestingly, in one TNBC patient, the same HPV DNA type was detected in the serum-derived EVs, cervical and BC tissue samples. Then, we report that HPV DNA can be transferred by EVs to recipient BC stromal cells that show an activated phenotype (e.g. CD44, IL6 expression) and an enhanced capability to sustain mammospheres (MS) formation. These data suggest that HPV DNA vehiculated by EVs is a potential trigger for BC niche aggressiveness.