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| Verfasst von: | Patry, Christian [VerfasserIn]  |
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| | Betzen, Christian [VerfasserIn] |
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| | Perez Ortiz, Alba [VerfasserIn] |
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| | Tönshoff, Burkhard [VerfasserIn] |
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| | Rafat, Neysan [VerfasserIn] |
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| Titel: | Metabolomic analysis of Shiga toxin 2a-induced injury in conditionally immortalized glomerular endothelial cells |
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| Verf.angabe: | Christian Patry, Kathrin Plotnicki, Christian Betzen, Alba Perez Ortiz, Kirk L. Pappan, Simon C. Satchell, Peter W. Mathieson, Martina Bielaszewska, Helge Karch, Burkhard Tönshoff, Neysan Rafat |
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| E-Jahr: | 2019 |
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| Jahr: | 01 October 2019 |
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| Fussnoten: | Gesehen am 29.10.2019 |
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| Titel Quelle: | Enthalten in: Metabolomics |
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| Ort Quelle: | Berlin : Springer, 2005 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 15(2019,10) Artikel-Nummer 131, 11 Seiten |
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| ISSN Quelle: | 1573-3890 |
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| Abstract: | IntroductionShiga toxin 2a (Stx2a) induces hemolytic uremic syndrome (STEC HUS) by targeting glomerular endothelial cells (GEC).ObjectivesWe investigated in a metabolomic analysis the response of a conditionally immortalized, stable glomerular endothelial cell line (ciGEnC) to Stx2a stimulation as a cell culture model for STEC HUS.MethodsCiGEnC were treated with tumor necrosis factor-(TNF)α, Stx2a or sequentially with TNFα and Stx2a. We performed a metabolomic high-throughput screening by lipid- or gas chromatography and subsequent mass spectrometry. Metabolite fold changes in stimulated ciGEnC compared to untreated cells were calculated.Results320 metabolites were identified and investigated. In response to TNFα + Stx2a, there was a predominant increase in intracellular free fatty acids and amino acids. Furthermore, lipid- and protein derived pro-inflammatory mediators, oxidative stress and an augmented intracellular energy turnover were increased in ciGEnC. Levels of most biochemicals related to carbohydrate metabolism remained unchanged.ConclusionStimulation of ciGEnC with TNFα + Stx2a is associated with profound metabolic changes indicative of increased inflammation, oxidative stress and energy turnover. |
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| DOI: | doi:10.1007/s11306-019-1594-2 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s11306-019-1594-2 |
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| | DOI: https://doi.org/10.1007/s11306-019-1594-2 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Conditionally immortalized glomerular endothelial cells |
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| | Hemolytic uremic syndrome |
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| | Metabolomics |
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| | Shiga toxin |
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| K10plus-PPN: | 1680556703 |
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| Verknüpfungen: | → Zeitschrift |
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Metabolomic analysis of Shiga toxin 2a-induced injury in conditionally immortalized glomerular endothelial cells / Patry, Christian [VerfasserIn]; 01 October 2019 (Online-Ressource)
