| |  Online-Ressource |
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| Verfasst von: | Klett, Hagen [VerfasserIn]  |
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| | Jürgensen, Lonny [VerfasserIn] |
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| | Most, Patrick [VerfasserIn] |
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| | Dobreva, Gergana [VerfasserIn] |
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| | Leuschner, Florian [VerfasserIn] |
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| | Hassel, David [VerfasserIn] |
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| | Busch, Martin [VerfasserIn] |
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| Titel: | Delineating the dynamic transcriptome response of mRNA and microRNA during zebrafish heart regeneration |
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| Verf.angabe: | Hagen Klett, Lonny Jürgensen, Patrick Most, Martin Busch, Fabian Günther, Gergana Dobreva, Florian Leuschner, David Hassel, Hauke Busch and Melanie Boerries |
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| Jahr: | 2019 |
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| Umfang: | 18 S. |
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| Fussnoten: | Published: 28 December 2018 ; Gesehen am 30.10.2019 |
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| Titel Quelle: | Enthalten in: Biomolecules |
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| Ort Quelle: | Basel : MDPI, 2011 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 9(2019,1) Artikel-Nummer 11, 18 Seiten |
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| ISSN Quelle: | 2218-273X |
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| Abstract: | Heart diseases are the leading cause of death for the vast majority of people around the world, which is often due to the limited capability of human cardiac regeneration. In contrast, zebrafish have the capacity to fully regenerate their hearts after cardiac injury. Understanding and activating these mechanisms would improve health in patients suffering from long-term consequences of ischemia. Therefore, we monitored the dynamic transcriptome response of both mRNA and microRNA in zebrafish at 1–160 days post cryoinjury (dpi). Using a control model of sham-operated and healthy fish, we extracted the regeneration specific response and further delineated the spatio-temporal organization of regeneration processes such as cell cycle and heart function. In addition, we identified novel (miR-148/152, miR-218b and miR-19) and previously known microRNAs among the top regulators of heart regeneration by using theoretically predicted target sites and correlation of expression profiles from both mRNA and microRNA. In a cross-species effort, we validated our findings in the dynamic process of rat myoblasts differentiating into cardiomyocytes-like cells (H9c2 cell line). Concluding, we elucidated different phases of transcriptomic responses during zebrafish heart regeneration. Furthermore, microRNAs showed to be important regulators in cardiomyocyte proliferation over time. |
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| DOI: | doi:10.3390/biom9010011 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/biom9010011 |
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| | Verlag: https://www.mdpi.com/2218-273X/9/1/11 |
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| | DOI: https://doi.org/10.3390/biom9010011 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cryoinjury |
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| | dynamic transcriptome |
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| | heart regeneration |
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| | miRNA |
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| | zebrafish |
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| K10plus-PPN: | 1680595342 |
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| Verknüpfungen: | → Zeitschrift |
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Delineating the dynamic transcriptome response of mRNA and microRNA during zebrafish heart regeneration / Klett, Hagen [VerfasserIn]; 2019 (Online-Ressource)
