| Online-Ressource |
Verfasst von: | Ghareeb, Mosad Ahmed [VerfasserIn]  |
| Sobeh, Mansour [VerfasserIn]  |
| Wink, Michael [VerfasserIn]  |
Titel: | Chemical profiling of polyphenolics in Eucalyptus globulus and evaluation of its hepato-renal protective potential against cyclophosphamide induced toxicity in mice |
Verf.angabe: | Mosad A. Ghareeb, Mansour Sobeh, Walaa H. El-Maadawy, Hala Sh Mohammed, Heba Khalil, Sanaa Botros and Michael Wink |
E-Jahr: | 2019 |
Jahr: | 19 September 2019 |
Umfang: | 19 S. |
Fussnoten: | Gesehen am 31.10.2019 |
Titel Quelle: | Enthalten in: Antioxidants |
Ort Quelle: | Basel : MDPI, 2013 |
Jahr Quelle: | 2019 |
Band/Heft Quelle: | 8(2019,9) Artikel-Nummer 415, 19 Seiten |
ISSN Quelle: | 2076-3921 |
Abstract: | Cyclophosphamide (CP) is a potent anti-neoplastic and immunosuppressive agent; however, it causes multi-organ toxicity. We elucidated the protective activities of Eucalyptus globulus (EG) leaf extract against CP-induced hepato–renal toxicity. Mice were treated with EG for 15 days plus CP on day 12 and 13 of the experiment. Using HPLC-DAD-ESI-MS/MS, 26 secondary metabolites were identified in EG leaf extract. Out of them, 4 polyphenolic compounds were isolated: (1) 4-(O-β-d-xylopyranosyloxy)-3,5-di-hydroxy-benzoic acid, (2) 4-(O-α-l-rhamnopyranosyloxy)-3,5-di-hydroxy-benzoic acid, (3) gallic acid, and (4) methyl gallate. Effects of EG extract on biochemical parameters, gene expression, and immune-histopathological changes were assessed in comparison to mesna positive control. Results showed that EG improved CP-increased serum ALT, AST, creatinine, and blood urea nitrogen levels. The hepatic and renal tissue levels of MDA, nitric oxide, protein carbonyl, TNF-α, IL-6, and immunohistochemical expression of nuclear factor kappa-B (NF-kB) and caspase-3 were reduced. Also, hepatic and renal GSH contents, and nuclear factor E2-related factor 2 (NRf2)/ hemoxygenase-1 (HO-1) signaling levels were increased. Histopathological findings supported our findings where hepatic and renal architecture were almost restored. Results revealed the protective effects of EG against CP-induced hepato–renal toxicity. These effects may be related to EG antioxidant, anti-inflammatory, and anti-apoptotic properties coupled with activation of Nrf2/HO-1 signaling. |
DOI: | doi:10.3390/antiox8090415 |
URL: | Volltext: https://doi.org/10.3390/antiox8090415 |
| Verlag: https://www.mdpi.com/2076-3921/8/9/415 |
| DOI: https://doi.org/10.3390/antiox8090415 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | <i>Eucalyptus globulus</i> |
| anti-inflammatory |
| antioxidant |
| cyclophosphamide |
| hepatotoxicity |
| nephrotoxicity |
| Nrf2/HO-1 signaling |
| polyphenolics |
K10plus-PPN: | 1680805053 |
Verknüpfungen: | → Zeitschrift |
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Lokale URL UB: | Zum Volltext |
Chemical profiling of polyphenolics in Eucalyptus globulus and evaluation of its hepato-renal protective potential against cyclophosphamide induced toxicity in mice / Ghareeb, Mosad Ahmed [VerfasserIn]; 19 September 2019 (Online-Ressource)