Chemical profiling of polyphenolics in Eucalyptus globulus and evaluation of its hepato-renal protective potential against cyclophosphamide induced toxicity in mice

• Verfasst von: Ghareeb, Mosad Ahmed [VerfasserIn]
• Verfasst von: Sobeh, Mansour [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Chemical profiling of polyphenolics in Eucalyptus globulus and evaluation of its hepato-renal protective potential against cyclophosphamide induced toxicity in mice
• Verf.angabe: Mosad A. Ghareeb, Mansour Sobeh, Walaa H. El-Maadawy, Hala Sh Mohammed, Heba Khalil, Sanaa Botros and Michael Wink
• E-Jahr: 2019
• Jahr: 19 September 2019
• Umfang: 19 S.
• Fussnoten: Gesehen am 31.10.2019
• Titel Quelle: Enthalten in: Antioxidants
• Ort Quelle: Basel : MDPI, 2013
• Jahr Quelle: 2019
• Band/Heft Quelle: 8(2019,9) Artikel-Nummer 415, 19 Seiten
• ISSN Quelle: 2076-3921
• DOI: doi:10.3390/antiox8090415
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: <i>Eucalyptus globulus</i>
• Sach-SW: anti-inflammatory
• Sach-SW: antioxidant
• Sach-SW: cyclophosphamide
• Sach-SW: hepatotoxicity
• Sach-SW: nephrotoxicity
• Sach-SW: Nrf2/HO-1 signaling
• Sach-SW: polyphenolics
• K10plus-PPN: 1680805053
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Cyclophosphamide (CP) is a potent anti-neoplastic and immunosuppressive agent; however, it causes multi-organ toxicity. We elucidated the protective activities of Eucalyptus globulus (EG) leaf extract against CP-induced hepato&ndash;renal toxicity. Mice were treated with EG for 15 days plus CP on day 12 and 13 of the experiment. Using HPLC-DAD-ESI-MS/MS, 26 secondary metabolites were identified in EG leaf extract. Out of them, 4 polyphenolic compounds were isolated: (1) 4-(O-&beta;-d-xylopyranosyloxy)-3,5-di-hydroxy-benzoic acid, (2) 4-(O-&alpha;-l-rhamnopyranosyloxy)-3,5-di-hydroxy-benzoic acid, (3) gallic acid, and (4) methyl gallate. Effects of EG extract on biochemical parameters, gene expression, and immune-histopathological changes were assessed in comparison to mesna positive control. Results showed that EG improved CP-increased serum ALT, AST, creatinine, and blood urea nitrogen levels. The hepatic and renal tissue levels of MDA, nitric oxide, protein carbonyl, TNF-&alpha;, IL-6, and immunohistochemical expression of nuclear factor kappa-B (NF-kB) and caspase-3 were reduced. Also, hepatic and renal GSH contents, and nuclear factor E2-related factor 2 (NRf2)/ hemoxygenase-1 (HO-1) signaling levels were increased. Histopathological findings supported our findings where hepatic and renal architecture were almost restored. Results revealed the protective effects of EG against CP-induced hepato&ndash;renal toxicity. These effects may be related to EG antioxidant, anti-inflammatory, and anti-apoptotic properties coupled with activation of Nrf2/HO-1 signaling.