| |  Online-Ressource |
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| Verfasst von: | Mitterer, Valentin [VerfasserIn]  |
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| | Shayan, Ramtin [VerfasserIn] |
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| | Ferreira-Cerca, Sébastien [VerfasserIn] |
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| | Murat, Guillaume [VerfasserIn] |
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| | Enne, Tanja [VerfasserIn] |
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| | Rinaldi, Dana [VerfasserIn] |
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| | Weigl, Sarah [VerfasserIn] |
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| | Omanic, Hajrija [VerfasserIn] |
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| | Gleizes, Pierre-Emmanuel [VerfasserIn] |
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| | Kressler, Dieter [VerfasserIn] |
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| | Plisson-Chastang, Celia [VerfasserIn] |
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| | Pertschy, Brigitte [VerfasserIn] |
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| Titel: | Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism |
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| Verf.angabe: | Valentin Mitterer, Ramtin Shayan, Sebastien Ferreira-Cerca, Guillaume Murat, Tanja Enne, Dana Rinaldi, Sarah Weigl, Hajrija Omanic, Pierre-Emmanuel Gleizes, Dieter Kressler, Celia Plisson-Chastang & Brigitte Pertschy |
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| E-Jahr: | 2019 |
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| Jahr: | 21 June 2019 |
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| Umfang: | 15 S. |
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| Fussnoten: | Published: 21 June 2019 ; Gesehen am 31.10.2019 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 10(2019) Artikel-Nummer 2754, 15 Seiten |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps. |
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| DOI: | doi:10.1038/s41467-019-10678-z |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
DOI: https://doi.org/10.1038/s41467-019-10678-z |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 90s |
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| | biogenesis |
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| | crystal-structure |
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| | eukaryotic ribosome |
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| | proteins |
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| | refinement |
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| | rna |
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| | system |
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| | translation initiation |
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| | transport |
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| K10plus-PPN: | 1680814338 |
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| Verknüpfungen: | → Zeitschrift |
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Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism / Mitterer, Valentin [VerfasserIn]; 21 June 2019 (Online-Ressource)
