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| Verfasst von: | Kempf, Georg [VerfasserIn]  |
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| | Stjepanović, Goran [VerfasserIn] |
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| | Sloan, Jeremy [VerfasserIn] |
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| | Hendricks, Astrid [VerfasserIn] |
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| | Lapouge, Karine [VerfasserIn] |
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| | Sinning, Irmgard [VerfasserIn] |
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| Titel: | The Escherichia coli SRP receptor forms a homodimer at the membrane |
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| Verf.angabe: | Georg Kempf, Goran Stjepanovic, Jeremy Sloan, Astrid Hendricks, Karine Lapouge, and Irmgard Sinning |
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| E-Jahr: | 2018 |
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| Jahr: | August 23, 2018 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 31.10.2019 |
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| Titel Quelle: | Enthalten in: Structure |
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| Ort Quelle: | London [u.a.] : Elsevier Science, 1993 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 26(2018), 11, Seite 1440-1450.e5 |
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| ISSN Quelle: | 1878-4186 |
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| Abstract: | The Escherichia coli signal recognition particle (SRP) receptor, FtsY, plays a fundamental role in co-translational targeting of membrane proteins via the SRP pathway. Efficient targeting relies on membrane interaction of FtsY and heterodimerization with the SRP protein Ffh, which is driven by detachment of α helix (αN1) in FtsY. Here we show that apart from the heterodimer, FtsY forms a nucleotide-dependent homodimer on the membrane, and upon αN1 removal also in solution. Homodimerization triggers reciprocal stimulation of GTP hydrolysis and occurs in vivo. Biochemical characterization together with integrative modeling suggests that the homodimer employs the same interface as the heterodimer. Structure determination of FtsY NG+1 with GMPPNP shows that a dimerization-induced conformational switch of the γ-phosphate is conserved in Escherichia coli, filling an important gap in SRP GTPase activation. Our findings add to the current understanding of SRP GTPases and may challenge previous studies that did not consider homodimerization of FtsY. |
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| DOI: | doi:10.1016/j.str.2018.07.008 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.str.2018.07.008 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0969212618302545 |
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| | DOI: https://doi.org/10.1016/j.str.2018.07.008 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | co-translational protein targeting |
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| | crosslinking |
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| | GTPase activation |
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| | membrane targeting sequence |
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| | MTS |
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| | SAXS |
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| | signal recognition particle |
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| | SRP |
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| | SRP GTPases |
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| | SRP receptor |
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| | X-ray structure determination |
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| K10plus-PPN: | 1680820605 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ Escherichia coli SRP receptor forms a homodimer at the membrane / Kempf, Georg [VerfasserIn]; August 23, 2018 (Online-Ressource)
