Status: Bibliographieeintrag
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| Verfasst von: | Sieber, Sandro [VerfasserIn]  |
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| | Uhl, Philipp [VerfasserIn] |
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| | Mier, Walter [VerfasserIn] |
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| Titel: | Zebrafish as a predictive screening model to assess macrophage clearance of liposomes in vivo |
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| Verf.angabe: | Sandro Sieber, Philip Grossen, Philipp Uhl, Pascal Detampel, Walter Mier, Dominik Witzigmann, Jörg Huwyler |
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| E-Jahr: | 2019 |
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| Jahr: | 17 January 2019 |
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| Umfang: | 12 S. |
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| Fussnoten: | Gesehen am 13.11.2019 |
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| Titel Quelle: | Enthalten in: Nanomedicine / Nanotechnology, biology and medicine |
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| Ort Quelle: | New York, NY : Elsevier, 2005 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 17(2019), Seite 82-93 |
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| ISSN Quelle: | 1549-9642 |
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| Abstract: | Macrophage recognition of nanoparticles is highly influenced by particle size and surface modification. Due to the lack of appropriate in vivo screening models, it is still challenging and time-consuming to characterize and optimize nanomedicines regarding this undesired clearance mechanism. Therefore, we validate zebrafish embryos as an emerging vertebrate screening tool to assess the macrophage sequestration of surface modified particulate formulations with varying particle size under realistic biological conditions. Liposomes with different PEG molecular weights (PEG350-PEG5000) at different PEG densities (3.0-10.0mol%) and particle sizes between 60 and 120nm were used as a well-established reference system showing various degrees of macrophage uptake. The results of in vitro experiments, zebrafish embryos, and in vivo rodent biodistribution studies were consistent, highlighting the validity of the newly introduced zebrafish macrophage clearance model. We hereby present a strategy for efficient, systematic and rapid nanomedicine optimization in order to facilitate the preclinical development of nanotherapeutics. |
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| DOI: | doi:10.1016/j.nano.2018.11.017 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.nano.2018.11.017 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S1549963419300115 |
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| | DOI: https://doi.org/10.1016/j.nano.2018.11.017 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cell uptake |
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| | Liposomes |
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| | Macrophage accumulation |
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| | Nanoparticles |
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| | PEG |
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| | Systemic clearance |
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| | Zebrafish screening model |
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| K10plus-PPN: | 1681807815 |
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| Verknüpfungen: | → Zeitschrift |
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Zebrafish as a predictive screening model to assess macrophage clearance of liposomes in vivo / Sieber, Sandro [VerfasserIn]; 17 January 2019 (Online-Ressource)
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