| |  Online-Ressource |
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| Verfasst von: | Affolter, Annette [VerfasserIn]  |
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| | Muller, Marie France [VerfasserIn] |
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| | Sommer, Katharina Sophia [VerfasserIn] |
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| | Stenzinger, Albrecht [VerfasserIn] |
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| | Zaoui, Karim [VerfasserIn] |
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| | Lorenz, Katja [VerfasserIn] |
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| | Wolf, Thomas [VerfasserIn] |
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| | Sharma, Sarika [VerfasserIn] |
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| | Wolf, Janina Luisa [VerfasserIn] |
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| | Weber, Klaus-Josef [VerfasserIn] |
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| | Freier, Kolja [VerfasserIn] |
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| | Plinkert, Peter K. [VerfasserIn] |
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| | Heß, Jochen [VerfasserIn] |
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| | Weichert, Wilko [VerfasserIn] |
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| Titel: | Targeting irradiation-induced mitogen-activated protein kinase activation in vitro and in an ex vivo model for human head and neck cancer |
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| Verf.angabe: | Annette Affolter, MD, Marie-France Muller, Katharina Sommer, Albrecht Stenzinger, MD, Karim Zaoui, MD, Katja Lorenz, Thomas Wolf, PhD, Sarika Sharma, PhD, Janina Wolf, Sven Perner, Klaus-Josef Weber, PhD, Kolja Freier, MD, Peter K. Plinkert, MD, Jochen Hess, PhD, Wilko Weichert, MD |
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| E-Jahr: | 2016 |
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| Jahr: | 26 February 2016 |
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| Umfang: | 13 S. |
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| Teil: | volume:38 |
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| | year:2016 |
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| | number:S1 |
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| | pages:E2049-E2061 |
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| | extent:13 |
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| Fussnoten: | Gesehen am 22.11.2019 |
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| Titel Quelle: | Enthalten in: Head & neck |
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| Ort Quelle: | New York, NY [u.a.] : Wiley Interscience, 1979 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 38(2016), S1, Seite E2049-E2061 |
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| ISSN Quelle: | 1097-0347 |
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| Abstract: | Background Despite new radiotherapeutic strategies, radioresistance in head and neck squamous cell carcinoma (HNSCC) remains a major problem. Preclinical model systems are needed to identify resistance mechanisms in this heterogeneous entity. Methods We elucidated the interplay among mitogen-activated protein kinase (MAPK)-inhibition, radiation, and p53 mutations in vitro and in a novel ex vivo model derived from vital human HNSCC samples. HNSCC cell lines (p53WT/mut) were treated with the mitogen-activated protein kinase (MEK)-inhibitor PD-0325901 and subsequently irradiated. Radiosensitization was functionally assessed and evaluated in the ex vivo model. Results We observed a pronounced irradiation-induced extracellular signal-regulated kinase (ERK) phosphorylation in 2 cell lines, which was independent of their p53 mutation status and associated with PD-0325901-related radiosensitization in a clonogenic assay. Heterogeneity in irradiation-induced ERK phosphorylation and in radiosensitization after MEK-inhibition was also reflected in the ex vivo model. Conclusion We provide experimental evidence for radiosensitizing effects of PD-0325901 in HNSCC. The ex vivo culture technology might offer a promising tool for individualized drug efficacy testing. © 2016 Wiley Periodicals, Inc. Head Neck 38: E2049-E2061, 2016 |
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| DOI: | doi:10.1002/hed.24376 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/hed.24376 |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/hed.24376 |
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| | DOI: https://doi.org/10.1002/hed.24376 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | ex vivo model |
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| | head and neck cancer |
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| | mitogen-activated protein (MAP) kinase signaling pathway |
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| | mitogen-activated protein kinase (MEK) inhibitor |
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| | p53 mutations |
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| | radioresistance |
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| K10plus-PPN: | 1682541525 |
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| Verknüpfungen: | → Zeitschrift |
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Targeting irradiation-induced mitogen-activated protein kinase activation in vitro and in an ex vivo model for human head and neck cancer / Affolter, Annette [VerfasserIn]; 26 February 2016 (Online-Ressource)
