Single-cell high-throughput technologies in cerebrospinal fluid research and diagnostics

• Verfasst von: Lanz, Tobias [VerfasserIn]
• Verfasst von: Pröbstel, Anne-Katrin [VerfasserIn]
• Verfasst von: Mildenberger, Iris [VerfasserIn]
• Verfasst von: Platten, Michael [VerfasserIn]
• Verfasst von: Schirmer, Lucas [VerfasserIn]
• Titel: Single-cell high-throughput technologies in cerebrospinal fluid research and diagnostics
• Verf.angabe: Tobias V. Lanz, Anne-Katrin Pröbstel, Iris Mildenberger, Michael Platten and Lucas Schirmer
• E-Jahr: 2019
• Jahr: 11 June 2019
• Fussnoten: Gesehen am 03.12.2019
• Titel Quelle: Enthalten in: Frontiers in immunology
• Ort Quelle: Lausanne : Frontiers Media, 2010
• Jahr Quelle: 2019
• Band/Heft Quelle: 10(2019) Artikel-Nummer 1302, 8 Seiten
• ISSN Quelle: 1664-3224
• DOI: doi:10.3389/fimmu.2019.01302
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cerebrospinal Fluid - CSF
• Sach-SW: Flow Cytometry
• Sach-SW: Mass spectromery
• Sach-SW: Repertoire sequencing
• Sach-SW: RNA sequencing (RNAseq)
• Sach-SW: Single cell gene expression
• K10plus-PPN: 1684103711
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

High-throughput single-cell technologies have recently emerged as essential tools in biomedical research with great potential for clinical pathology when studying liquid and solid biopsies. We provide an update on current single-cell methods in cerebrospinal fluid research and diagnostics focusing on high-throughput cell-type specific proteomic and genomic technologies. Proteomic methods comprising flow cytometry and mass cytometry as well as genomic approaches including immune cell repertoire and single-cell transcriptomic studies are critically reviewed and future directions discussed.