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Verfasst von:Kazemi Noureini, Sakineh [VerfasserIn]   i
 Fatemi, Leili [VerfasserIn]   i
 Wink, Michael [VerfasserIn]   i
Titel:Telomere shortening in breast cancer cells (MCF7) under treatment with low doses of the benzylisoquinoline alkaloid chelidonine
Verf.angabe:Sakineh Kazemi Noureini, Leili Fatemi, Michael Wink
E-Jahr:2018
Jahr:October 3, 2018
Umfang:15 S.
Illustrationen:Diagramme, Illustrationen
Fussnoten:Gesehen am 04.12.2019
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2018
Band/Heft Quelle:13(2018,10) Artikel-Nummer e0204901, Seite 1-15, 15 Seiten
ISSN Quelle:1932-6203
Abstract:Telomeres, the specialized dynamic structures at chromosome ends, regularly shrink with every replication. Thus, they function as an internal molecular clock counting down the number of cell divisions. However, most cancer cells escape this limitation by activating telomerase, which can maintain telomere length. Previous studies showed that the benzylisoquinoline alkaloid chelidonine stimulates multiple modes of cell death and strongly down-regulates telomerase. It is still unknown if down-regulation of telomerase by chelidonine boosts substantial telomere shortening. The breast cancer cell line MCF7 was sequentially treated with very low concentrations of chelidonine over several cell passages. Telomere length and telomerase activity were measured by a monochrome multiplex quantitative PCR and a q-TRAP assay, respectively. Changes in population size and doubling time correlated well with telomerase inhibition and telomere shortening. MCF7 cell growth was arrested completely after three sequential treatments with 0.1 μM chelidonine, each ending after 48 h, while telomere length was reduced to almost 10% of the untreated control. However, treatment with 0.01 μM chelidonine did not have any apparent consequence. In addition to dose and time dependent telomerase inhibition, chelidonine changed the splicing pattern of hTERT towards non-enzyme coding isoforms of the transcript. In conclusion, telomere length and telomere stability are strongly affected by chelidonine in addition to microtubule formation.
DOI:doi:10.1371/journal.pone.0204901
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0204901
 Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0204901
 DOI: https://doi.org/10.1371/journal.pone.0204901
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Alkaloids
 Cancer treatment
 Cell growth
 Chromosome structure and function
 Non-homologous end joining
 Polymerase chain reaction
 Telomere length
 Telomeres
K10plus-PPN:1684203015
Verknüpfungen:→ Zeitschrift

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