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Verfasst von:Hösl, Michaela [VerfasserIn]   i
 Kleimaier, Dennis [VerfasserIn]   i
 Hu, Ruomin [VerfasserIn]   i
 Malzacher, Matthias [VerfasserIn]   i
 Nies, Cordula [VerfasserIn]   i
 Gottwald, Eric [VerfasserIn]   i
 Schad, Lothar R. [VerfasserIn]   i
Titel:23Na triple-quantum signal of in vitro human liver cells, liposomes, and nanoparticles
Titelzusatz:Cell viability assessment vs. separation of intra- and extracellular signal
Verf.angabe:Michaela A.U. Hoesl, MS, Dennis Kleimaier, MS, Ruomin Hu, MS, Matthias Malzacher, MS, Cordula Nies, Eric Gottwald, PhD, and Lothar R. Schad, PhD
E-Jahr:2019
Jahr:25 January 2019
Umfang:10 S.
Fussnoten:Im Titel ist die Zahl "23" hochgestellt ; Gesehen am 05.12.2019
Titel Quelle:Enthalten in: Journal of magnetic resonance imaging
Ort Quelle:New York, NY : Wiley-Liss, 1991
Jahr Quelle:2019
Band/Heft Quelle:50(2019), 2, Seite 435-444
ISSN Quelle:1522-2586
Abstract:Background Triple-quantum (TQ) filtered sequences have become more popular in sodium MR due to the increased usage of scanners with field strengths exceeding 3T. Disagreement as to whether TQ signal can provide separation of intra- and extracellular compartments persists. Purpose To provide insight into TQ signal behavior on a cellular level. Study Type Prospective. Phantom/Specimen Cell-phantoms in the form of liposomes, encapsulated 0 mM, 145 mM, 154 mM Na+ in a double-lipid membrane similar to cells. Poly(lactic-co-glycolic acid) nanoparticles encapsulated 154 mM Na+ within a single-layer membrane structure. Two microcavity chips with each 6 × 106 human HEP G2 liver cells were measured in an MR-compatible bioreactor. Field Strength/Sequence Spectroscopic TQ sequence with time proportional phase-increments at 9.4T. Assessment The TQ signal of viable, dead cells, and cell-phantoms was assessed by a fit in the time domain and by the amplitude in the frequency domain. Statistical Tests The noise variance (σ) was evaluated to express the deviation of the measured TQ signal amplitude from noise. Results TQ signal >20σ was found for liposomes encapsulating sodium ions. Liposomal encapsulation of 0 mM Na+ and 154 mM Na+ encapsulation in the nanoparticles resulted in <2σ TQ signal. Cells under normal perfusion resulted in >9σ TQ signal. Compared with TQ signal under normal perfusion, a 56% lower TQ signal of was observed (25σ) during perfusion stop. TQ signal returned to 92% of the initial signal after reperfusion. Data Conclusion Our measurements indicate that TQ signal in liposomes was observed due to the trapping of ions within the double-lipid membrane rather than from the intraliposomal space. Transfer to the cell results suggests that TQ signal was observed from motion restriction equivalent to trapping. Level of Evidence: 1 Technical Efficacy: Stage 3 J. Magn. Reson. Imaging 2019;50:435-444.
DOI:doi:10.1002/jmri.26666
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1002/jmri.26666
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/jmri.26666
 DOI: https://doi.org/10.1002/jmri.26666
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:sodium NMR
 sodium triple-quantum signal
 spin 3/2
 TQTPPI
K10plus-PPN:1684394279
Verknüpfungen:→ Zeitschrift

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