miR-339-3p is a tumor suppressor in melanoma

• Verfasst von: Weber, Claudia [VerfasserIn]
• Verfasst von: Luo, Chonglin [VerfasserIn]
• Verfasst von: Hotz-Wagenblatt, Agnes [VerfasserIn]
• Verfasst von: Gardyan, Adriane [VerfasserIn]
• Verfasst von: Kordaß, Theresa [VerfasserIn]
• Verfasst von: Holland-Letz, Tim [VerfasserIn]
• Verfasst von: Osen, Wolfram [VerfasserIn]
• Verfasst von: Eichmüller, Stefan B. [VerfasserIn]
• Titel: miR-339-3p is a tumor suppressor in melanoma
• Verf.angabe: Claudia E.M. Weber, Chonglin Luo, Agnes Hotz-Wagenblatt, Adriane Gardyan, Theresa Kordaß, Tim Holland-Letz, Wolfram Osen, and Stefan B. Eichmüller
• E-Jahr: 2016
• Jahr: April 15, 2016
• Umfang: 10 S.
• Fussnoten: Gesehen am 13.12.2019
• Titel Quelle: Enthalten in: Cancer research
• Ort Quelle: Philadelphia, Pa. : AACR, 1916
• Jahr Quelle: 2016
• Band/Heft Quelle: 76(2016), 12, Seite 3562-3571
• ISSN Quelle: 1538-7445
• DOI: doi:10.1158/0008-5472.CAN-15-2932
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1685501702

Abstract

Determinants of invasion and metastasis in cancer remain of great interest to define. Here, we report the definition of miR-339-3p as a novel tumor suppressive microRNA that blocks melanoma cell invasion without affecting cell survival. miR-339-3p was identified by a comprehensive functional screen of a human miRNA mimetic library in a cell-based assay for invasion by the melanoma cell line A375. miR-339-3p was determined as a strong inhibitor of invasion differentially expressed in melanoma cells and healthy melanocytes. MCL1 was defined as a target for downregulation by miR-339-3p, functioning through direct interaction with the 3′ untranslated region of MCL1 mRNA. Blocking miR-339-3p by an antagomiR was sufficient to increase melanoma cell invasion, an effect that could be phenocopied by RNAi-mediated silencing of MCL1. In vivo studies established that miR-339-3p overexpression was sufficient to decrease lung colonization by A375 melanoma cells in NSG mice, relative to control cells. Overall, our results defined miR-339-3p as a melanoma tumor suppressor, the levels of which contributes to invasive aggressiveness.