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| Verfasst von: | Sobeh, Mansour [VerfasserIn]  |
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| | Youssef, Fadia S. [VerfasserIn] |
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| | Esmat, Ahmed [VerfasserIn] |
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| | Petruk, Ganna [VerfasserIn] |
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| | El-Khatib, Ahmed H. [VerfasserIn] |
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| | Monti, Daria Maria [VerfasserIn] |
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| | Ashour, Mohamed L. [VerfasserIn] |
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| | Wink, Michael [VerfasserIn] |
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| Titel: | High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage |
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| Verf.angabe: | Mansour Sobeh, Fadia S. Youssef, Ahmed Esmat, Ganna Petruk, Ahmed H. El-Khatib, Daria Maria Monti, Mohamed L. Ashour, Michael Wink |
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| E-Jahr: | 2018 |
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| Jahr: | 31 January 2018 |
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| Umfang: | 9 S. |
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| Fussnoten: | Gesehen am 17.12.2019 ; Im Titel ist die Zahl 4 tiefgestellt dargestellt ; Syzygium samarangense ist kursiv dargestellt |
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| Titel Quelle: | Enthalten in: Food and chemical toxicology |
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| Ort Quelle: | New York, NY [u.a.] : Elsevier, 1982 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 113(2018), Seite 145-153 |
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| ISSN Quelle: | 1873-6351 |
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| Abstract: | Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug. |
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| DOI: | doi:10.1016/j.fct.2018.01.031 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.fct.2018.01.031 |
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| | Verlag: http://www.sciencedirect.com/science/article/pii/S0278691518300310 |
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| | DOI: https://doi.org/10.1016/j.fct.2018.01.031 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Antioxidant |
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| | Carbon tetrachloride toxicity |
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| | HaCaT cells |
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| | Hepatoprotective activity |
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| | HR-UPLC-MS/MS |
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| K10plus-PPN: | 1685778119 |
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| Verknüpfungen: | → Zeitschrift |
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High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage / Sobeh, Mansour [VerfasserIn]; 31 January 2018 (Online-Ressource)
