Functional characterization of a drought-responsive invertase inhibitor from Maize (Zea mays L.)

• Verfasst von: Chen, Lin [VerfasserIn]
• Verfasst von: Greiner, Steffen [VerfasserIn]
• Verfasst von: Rausch, Thomas [VerfasserIn]
• Titel: Functional characterization of a drought-responsive invertase inhibitor from Maize (Zea mays L.)
• Verf.angabe: Lin Chen, Xiaohong Liu, Xiaojia Huang, Wei Luo, Yuming Long, Steffen Greiner, Thomas Rausch, Hongbo Zhao
• E-Jahr: 2019
• Jahr: 21 August 2019
• Umfang: 18 S.
• Fussnoten: Gesehen am 23.12.2019
• Titel Quelle: Enthalten in: International journal of molecular sciences
• Ort Quelle: Basel : Molecular Diversity Preservation International, 2000
• Jahr Quelle: 2019
• Band/Heft Quelle: 20(2019,17) Artikel-Nummer 4081, Seite 1-18, 18 Seiten
• ISSN Quelle: 1422-0067
• ISSN Quelle: 1661-6596
• DOI: doi:10.3390/ijms20174081
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: <i>Zea mays</i>
• Sach-SW: abscisic acid (ABA)
• Sach-SW: drought
• Sach-SW: invertase inhibitor
• Sach-SW: regulation
• K10plus-PPN: 1686323964

Abstract

Invertases (INVs) play essential roles in plant growth in response to environmental cues. Previous work showed that plant invertases can be post-translationally regulated by small protein inhibitors (INVINHs). Here, this study characterizes a proteinaceous inhibitor of INVs in maize (Zm-INVINH4). A functional analysis of the recombinant Zm-INVINH4 protein revealed that it inhibited both cell wall and vacuolar invertase activities from maize leaves. A Zm-INVINH4::green fluorescent protein fusion experiment indicated that this protein localized in the apoplast. Transcript analysis showed that Zm-INVINH4 is specifically expressed in maize sink tissues, such as the base part of the leaves and young kernels. Moreover, drought stress perturbation significantly induced Zm-INVINH4 expression, which was accompanied with a decrease of cell wall invertase (CWI) activities and an increase of sucrose accumulation in both base parts of the leaves 2 to 7 days after pollinated kernels. In summary, the results support the hypothesis that INV-related sink growth in response to drought treatment is (partially) caused by a silencing of INV activity via drought-induced induction of Zm-INVINH4 protein.