Noncontact recognition of fluorescently labeled objects in deep tissue via a novel optical light beam arrangement

• Verfasst von: Hien, Andreas [VerfasserIn]
• Verfasst von: Pretze, Marc [VerfasserIn]
• Verfasst von: Braun, Frank [VerfasserIn]
• Verfasst von: Schäfer, Edgar [VerfasserIn]
• Verfasst von: Kümmel, Tim [VerfasserIn]
• Verfasst von: Roscher, Mareike [VerfasserIn]
• Verfasst von: Schock-Kusch, Daniel [VerfasserIn]
• Verfasst von: Waldeck, Jens [VerfasserIn]
• Verfasst von: Müller, Bernhard [VerfasserIn]
• Verfasst von: Wängler, Carmen [VerfasserIn]
• Verfasst von: Rädle, Matthias [VerfasserIn]
• Verfasst von: Wängler, Björn [VerfasserIn]
• Titel: Noncontact recognition of fluorescently labeled objects in deep tissue via a novel optical light beam arrangement
• Verf.angabe: Andreas Hien, Marc Pretze, Frank Braun, Edgar Schäfer, Tim Kümmel, Mareike Roscher, Daniel Schock-Kusch, Jens Waldeck, Bernhard Müller, Carmen Wängler, Matthias Rädle, Björn Wängler
• E-Jahr: 2018
• Jahr: December 19, 2018
• Umfang: 18 S.
• Fussnoten: Published: December 19, 2018 ; Gesehen am 14.01.2020
• Titel Quelle: Enthalten in: PLOS ONE
• Ort Quelle: San Francisco, California, US : PLOS, 2006
• Jahr Quelle: 2018
• Band/Heft Quelle: 13(2018,12) Artikel-Nummer e0208236, 18 Seiten
• ISSN Quelle: 1932-6203
• DOI: doi:10.1371/journal.pone.0208236
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Artificial light
• Sach-SW: Bright field imaging
• Sach-SW: Fluorescence
• Sach-SW: Fluorescence imaging
• Sach-SW: In vivo imaging
• Sach-SW: Lasers
• Sach-SW: Light
• Sach-SW: Magnetic resonance imaging
• K10plus-PPN: 1687304971

Abstract

To date, few optical imaging systems are available in clinical practice to perform noninvasive measurements transcutaneously. Instead, functional imaging is performed using ionizing radiation or intense magnetic fields in most cases. The applicability of fluorescence imaging (e.g., for the detection of fluorescently labeled objects, such as tumors) is limited due to the restricted tissue penetration of light and the required long exposure time. Thus, the development of highly sensitive and easily manageable instruments is necessary to broaden the utility of optical imaging. To advance these developments, an improved fluorescence imaging system was designed in this study that operates on the principle of noncontact laser-induced fluorescence and enables the detection of fluorescence from deeper tissue layers as well as real-time imaging. The high performance of the developed optical laser scanner results from the combination of specific point illumination, an intensified charge-coupled device (ICCD) detector with a novel light trap, and a filtering strategy. The suitability of the laser scanner was demonstrated in two representative applications and an in vivo evaluation. In addition, a comparison with a planar imaging system was performed. The results show that the exposure time with the developed laser scanner can be reduced to a few milliseconds during measurements with a penetration depth of up to 32 mm. Due to these short exposure times, real-time fluorescence imaging can be easily achieved. The ability to measure fluorescence from deep tissue layers enables clinically relevant applications, such as the detection of fluorescently labeled malignant tumors.