Endothelial progenitor and mesenchymal stromal cells in newborns with congenital diaphragmatic hernia undergoing extracorporeal membrane oxygenation

• Verfasst von: Rafat, Neysan [VerfasserIn]
• Verfasst von: Patry, Christian [VerfasserIn]
• Verfasst von: Sabet, Ursula [VerfasserIn]
• Verfasst von: Viergutz, Tim [VerfasserIn]
• Verfasst von: Weiß, Christel [VerfasserIn]
• Verfasst von: Tönshoff, Burkhard [VerfasserIn]
• Verfasst von: Beck, Grietje [VerfasserIn]
• Verfasst von: Schaible, Thomas [VerfasserIn]
• Titel: Endothelial progenitor and mesenchymal stromal cells in newborns with congenital diaphragmatic hernia undergoing extracorporeal membrane oxygenation
• Verf.angabe: Neysan Rafat, Christian Patry, Ursula Sabet, Tim Viergutz, Christel Weiss, Burkhard Tönshoff, Grietje Beck and Thomas Schaible
• E-Jahr: 2019
• Jahr: 22 November 2019
• Umfang: 9 S.
• Fussnoten: Gesehen am 14.01.2020
• Titel Quelle: Enthalten in: Frontiers in Pediatrics
• Ort Quelle: Lausanne : Frontiers Media, 2013
• Jahr Quelle: 2019
• Band/Heft Quelle: 7(2019) Artikel-Nummer 490, 9 Seiten
• ISSN Quelle: 2296-2360
• DOI: doi:10.3389/fped.2019.00490
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Errata: Rafat, Neysan, 1979 - : Corrigendum: Endothelial progenitor and mesenchymal stromal cells in newborns with congenital diaphragmatic hernia undergoing extracorporeal membrane oxygenation
• Sach-SW: Congenital Diaphragmatic Hernia
• Sach-SW: ECMO
• Sach-SW: neonates
• Sach-SW: progenitor cells
• Sach-SW: Stem Cells
• K10plus-PPN: 1687324247

Abstract

Background: Endothelial progenitor (EPC) and mesenchymal stromal cells (MSC) can regenerate damaged endothelium and thereby improve pulmonary endothelial dysfunction. We do not know, how extracorporeal membrane oxygenation (ECMO) might affect EPC- and MSC-mediated regenerative pathways in patients with congenital diaphragmatic hernia (CDH). Therefore, we investigated, if ECMO support impacts EPC and MSC numbers in CDH patients. Methods: Peripheral blood mononuclear cells from newborns with ECMO-dependent (n=18) and ECMO-independent CDH (n=12) and from healthy controls (n=12) were isolated. The number of EPC and MSC was identified by flowcytometry. Serum levels of vascular endothelial growth factor (VEGF) and angiopoietin (Ang)-2 were determined. Results: EPC and MSC were elevated in newborns with CDH. ECMO-dependent infants had higher EPC subpopulation counts (2,1-7,6-fold) before treatment compared to ECMO-independent infants. In the disease course, EPC and MSC subpopulation counts in ECMO-dependent infants were lower than before ECMO initiation. During ECMO, VEGF serum levels were significantly reduced (by 90,5%) and Ang2 levels significantly increased (by 74,8%). Conclusions: Our data suggest that ECMO might be associated with a rather impaired mobilization of EPC and MSC and with a depression of VEGF serum levels in newborns with CDH.