Web-based design and analysis tools for CRISPR base editing

• Verfasst von: Hwang, Gue-Ho [VerfasserIn]
• Verfasst von: Park, Jeongbin [VerfasserIn]
• Verfasst von: Eils, Roland [VerfasserIn]
• Titel: Web-based design and analysis tools for CRISPR base editing
• Verf.angabe: Gue-Ho Hwang, Jeongbin Park, Kayeong Lim, Sunghyun Kim, Jihyeon Yu, Eunchong Yu, Sang-Tae Kim, Roland Eils, Jin-Soo Kim, Sangsu Bae
• E-Jahr: 2018
• Jahr: 27 December 2018
• Umfang: 7 S.
• Fussnoten: Gesehen am 23.01.2020
• Titel Quelle: Enthalten in: BMC bioinformatics
• Ort Quelle: London : BioMed Central, 2000
• Jahr Quelle: 2018
• Band/Heft Quelle: 19(2018) Artikel-Nr. 542, 7 Seiten
• ISSN Quelle: 1471-2105
• DOI: doi:10.1186/s12859-018-2585-4
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1688123512

Abstract

As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which consist of deactivated Cas9 (dCas9) or Cas9 nickase (nCas9) linked with a cytidine or a guanine deaminase, have been developed. Base editing tools will be very useful for gene correction because they can produce highly specific DNA substitutions without the introduction of any donor DNA, but dedicated web-based tools to facilitate the use of such tools have not yet been developed.