| |  Online-Ressource |
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| Verfasst von: | Serçin, Özdemirhan [VerfasserIn]  |
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| | Rein, Katrin [VerfasserIn] |
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| | Halavatyi, Aliaksandr [VerfasserIn] |
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| | Winter, Hauke [VerfasserIn] |
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| | Abdollahi, Amir [VerfasserIn] |
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| Titel: | A solid-phase transfection platform for arrayed CRISPR screens |
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| Verf.angabe: | Özdemirhan Serçin, Sabine Reither, Paris Roidos, Nadja Ballin, Spyridon Palikyras, Anna Baginska, Katrin Rein, Maria Llamazares, Aliaksandr Halavatyi, Hauke Winter, Thomas Muley, Renata Z. Jurkowska, Amir Abdollahi, Frank T. Zenke, Beate Neumann & Balca R. Mardin |
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| E-Jahr: | 2019 |
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| Jahr: | 23 December 2019 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 24.01.2020 |
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| Titel Quelle: | Enthalten in: Molecular systems biology |
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| Ort Quelle: | Heidelberg : EMBO Press, 2005 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 15(2019,12) Artikel-Nummer e8983, 13 Seiten |
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| ISSN Quelle: | 1744-4292 |
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| Abstract: | Abstract Arrayed CRISPR-based screens emerge as a powerful alternative to pooled screens making it possible to investigate a wide range of cellular phenotypes that are typically not amenable to pooled screens. Here, we describe a solid-phase transfection platform that enables CRISPR-based genetic screens in arrayed format with flexible readouts. We demonstrate efficient gene knockout upon delivery of guide RNAs and Cas9/guide RNA ribonucleoprotein complexes into untransformed and cancer cell lines. In addition, we provide evidence that our platform can be easily adapted to high-throughput screens and we use this approach to study oncogene addiction in tumor cells. Finally demonstrating that the human primary cells can also be edited using this method, we pave the way for rapid testing of potential targeted therapies. |
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| DOI: | doi:10.15252/msb.20198983 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.15252/msb.20198983 |
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| | Verlag: https://www.embopress.org/doi/full/10.15252/msb.20198983 |
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| | DOI: https://doi.org/10.15252/msb.20198983 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | arrayed screens |
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| | CRISPR/Cas9 |
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| | gRNA/RNP delivery |
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| | solid phase |
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| | transfection platform |
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| K10plus-PPN: | 1688467874 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ solid-phase transfection platform for arrayed CRISPR screens / Serçin, Özdemirhan [VerfasserIn]; 23 December 2019 (Online-Ressource)
