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Verfasst von:Hirsch, Burkhard [VerfasserIn]   i
 Endris, Volker [VerfasserIn]   i
 Laßmann, Silke [VerfasserIn]   i
 Weichert, Wilko [VerfasserIn]   i
 Pfarr, Nicole [VerfasserIn]   i
 Schirmacher, Peter [VerfasserIn]   i
 Kovaleva, Valentina [VerfasserIn]   i
 Werner, Martin [VerfasserIn]   i
 Bonzheim, I. [VerfasserIn]   i
 Fend, Falko [VerfasserIn]   i
 Sperveslage, Jan [VerfasserIn]   i
 Kaulich, K. [VerfasserIn]   i
 Zacher, A. [VerfasserIn]   i
 Reifenberger, Guido [VerfasserIn]   i
 Köhrer, Karl [VerfasserIn]   i
 Stepanow, S. [VerfasserIn]   i
 Lerke, S. [VerfasserIn]   i
 Mayr, T. [VerfasserIn]   i
 Aust, D. E. [VerfasserIn]   i
 Baretton, Gustavo Bruno [VerfasserIn]   i
 Weidner, S. [VerfasserIn]   i
 Jung, A. [VerfasserIn]   i
 Kirchner, T. [VerfasserIn]   i
 Hansmann, M. L. [VerfasserIn]   i
 Burbat, L. [VerfasserIn]   i
 von der Wall, E. [VerfasserIn]   i
 Dietel, Manfred [VerfasserIn]   i
 Hummel, M. [VerfasserIn]   i
Titel:Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics
Verf.angabe:B. Hirsch, V. Endris, S. Lassmann, W. Weichert, N. Pfarr, P. Schirmacher, V. Kovaleva, M. Werner, I. Bonzheim, F. Fend, J. Sperveslage, K. Kaulich, A. Zacher, G. Reifenberger, K. Köhrer, S. Stepanow, S. Lerke, T. Mayr, D. E. Aust, G. Baretton, S. Weidner, A. Jung, T. Kirchner, M. L. Hansmann, L. Burbat, E. von der Wall, M. Dietel, M. Hummel
E-Jahr:2018
Jahr:27 January 2018
Umfang:9 S.
Fussnoten:First online: 27 January 2018 ; Gesehen am 27.01.2020
Titel Quelle:Enthalten in: Virchows Archiv
Ort Quelle:Berlin : Springer, 1847
Jahr Quelle:2018
Band/Heft Quelle:472(2018), 4, Seite 557-565
ISSN Quelle:1432-2307
Abstract:The simultaneous detection of multiple somatic mutations in the context of molecular diagnostics of cancer is frequently performed by means of amplicon-based targeted next-generation sequencing (NGS). However, only few studies are available comparing multicenter testing of different NGS platforms and gene panels. Therefore, seven partner sites of the German Cancer Consortium (DKTK) performed a multicenter interlaboratory trial for targeted NGS using the same formalin-fixed, paraffin-embedded (FFPE) specimen of molecularly pre-characterized tumors (n = 15; each n = 5 cases of Breast, Lung, and Colon carcinoma) and a colorectal cancer cell line DNA dilution series. Detailed information regarding pre-characterized mutations was not disclosed to the partners. Commercially available and custom-designed cancer gene panels were used for library preparation and subsequent sequencing on several devices of two NGS different platforms. For every case, centrally extracted DNA and FFPE tissue sections for local processing were delivered to each partner site to be sequenced with the commercial gene panel and local bioinformatics. For cancer-specific panel-based sequencing, only centrally extracted DNA was analyzed at seven sequencing sites. Subsequently, local data were compiled and bioinformatics was performed centrally. We were able to demonstrate that all pre-characterized mutations were re-identified correctly, irrespective of NGS platform or gene panel used. However, locally processed FFPE tissue sections disclosed that the DNA extraction method can affect the detection of mutations with a trend in favor of magnetic bead-based DNA extraction methods. In conclusion, targeted NGS is a very robust method for simultaneous detection of various mutations in FFPE tissue specimens if certain pre-analytical conditions are carefully considered.
DOI:doi:10.1007/s00428-017-2288-7
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1007/s00428-017-2288-7
 DOI: https://doi.org/10.1007/s00428-017-2288-7
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Amplicon-based next-generation sequencing
 FFPE cancer samples
 German Cancer Research Centers (DKTK-sites)
 MiSeq™
 Multicenter trial
 PGM™
K10plus-PPN:1688550860
Verknüpfungen:→ Zeitschrift

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