Status: Bibliographieeintrag
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| Verfasst von: | Al-Shehabi, Hussein [VerfasserIn]  |
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| | Kutzner, Juliane [VerfasserIn] |
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| | Schaller, Torsten [VerfasserIn] |
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| Titel: | Human SAMHD1 restricts the xenotransplantation relevant porcine endogenous retrovirus (PERV) in non-dividing cells |
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| Verf.angabe: | Hussein Al-Shehabi, Uwe Fiebig, Juliane Kutzner, Joachim Denner, Torsten Schaller, Norbert Bannert, Henning Hofmann |
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| E-Jahr: | 2019 |
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| Jahr: | 15 February 2019 |
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| Umfang: | 6 S. |
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| Fussnoten: | Gesehen am 29.01.2020 |
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| Titel Quelle: | Enthalten in: Journal of general virology |
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| Ort Quelle: | Reading : Soc., 1967 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 100(2019), 4, Seite 656-661 |
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| ISSN Quelle: | 1465-2099 |
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| Abstract: | The release of porcine endogenous retrovirus (PERV) particles from pig cells is a potential risk factor during xenotransplantation by way of productively infecting the human transplant recipient. Potential countermeasures against PERV replication are restriction factors that block retroviral replication. SAMHD1 is a triphosphohydrolase that depletes the cellular pool of dNTPs in non-cycling cells starving retroviral reverse transcription. We investigated the antiviral activity of human SAMHD1 against PERV and found that SAMHD1 potently restricts its reverse transcription in human monocytes, monocyte-derived dendritic cells (MDDC), or macrophages (MDM) and in monocytic THP-1 cells. Degradation of SAMHD1 by SIVmac Vpx or CRISPR/Cas9 knock-out of SAMHD1 allowed for PERV reverse transcription. Addition of deoxynucleosides alleviated the SAMHD1-mediated restriction suggesting that SAMHD1-mediated degradation of dNTPs restricts PERV replication in these human immune cells. In conclusion, our findings highlight SAMHD1 as a potential barrier to PERV transmission from pig transplants to human recipients during xenotransplantation., |
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| DOI: | doi:10.1099/jgv.0.001232 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1099/jgv.0.001232 |
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| | Verlag: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001232 |
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| | DOI: https://doi.org/10.1099/jgv.0.001232 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1688790829 |
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| Verknüpfungen: | → Zeitschrift |
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Human SAMHD1 restricts the xenotransplantation relevant porcine endogenous retrovirus (PERV) in non-dividing cells / Al-Shehabi, Hussein [VerfasserIn]; 15 February 2019 (Online-Ressource)
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