| |  Online-Ressource |
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| Verfasst von: | Kassianidou, Elena [VerfasserIn]  |
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| | Probst, Dimitri [VerfasserIn] |
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| | Jäger, Julia [VerfasserIn] |
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| | Schwarz, Ulrich S. [VerfasserIn] |
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| Titel: | Extracellular matrix geometry and initial adhesive position determine stress fiber network organization during cell spreading |
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| Verf.angabe: | Elena Kassianidou, Dimitri Probst, Julia Jäger, Stacey Lee, Anne-Lou Roguet, Ulrich Sebastian Schwarz, and Sanjay Kumar |
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| E-Jahr: | 2019 |
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| Jahr: | 7 May 2019 |
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| Umfang: | 18 S. |
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| Fussnoten: | Gesehen am 17.02.2020 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 27(2019), 6, Seite 1897-1909,e1-e4 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Three-dimensional matrices often contain highly structured adhesive tracks that require cells to turn corners and bridge non-adhesive areas. Here, we investigate these complex processes using micropatterned cell adhesive frames. Spreading kinetics on these matrices depend strongly on initial adhesive position and are predicted by a cellular Potts model (CPM), which reflects a balance between adhesion and intracellular tension. As cells spread, new stress fibers (SFs) assemble periodically and parallel to the leading edge, with spatial intervals of ∼2.5 μm, temporal intervals of ∼15 min, and characteristic lifetimes of ∼50 min. By incorporating these rules into the CPM, we can successfully predict SF network architecture. Moreover, we observe broadly similar behavior when we culture cells on arrays of discrete collagen fibers. Our findings show that ECM geometry and initial cell position strongly determine cell spreading and that cells encode a memory of their spreading history through SF network organization. |
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| DOI: | doi:10.1016/j.celrep.2019.04.035 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.celrep.2019.04.035 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S2211124719305005 |
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| | DOI: https://doi.org/10.1016/j.celrep.2019.04.035 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | actin cytoskeleton |
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| | cell memory |
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| | cell migration |
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| | cell shape |
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| | cell spreading |
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| | cell-matrix adhesion |
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| | cellular Potts model |
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| | mathematical modeling |
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| | mechanobiology |
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| | stress fibers |
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| K10plus-PPN: | 1690138173 |
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| Verknüpfungen: | → Zeitschrift |
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Extracellular matrix geometry and initial adhesive position determine stress fiber network organization during cell spreading / Kassianidou, Elena [VerfasserIn]; 7 May 2019 (Online-Ressource)
