Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Scherthan, Harry [VerfasserIn]  |
| Chojowski, Robert [VerfasserIn]  |
| Hausmann, Michael [VerfasserIn]  |
Titel: | Nanostructure of clustered DNA damage in leukocytes after in-solution irradiation with the alpha emitter Ra-223 |
Verf.angabe: | Harry Scherthan, Jin-Ho Lee, Emanuel Maus, Sarah Schumann, Razan Muhtadi, Robert Chojowski, Matthias Port, Michael Lassmann, Felix Bestvater and Michael Hausmann |
E-Jahr: | 2019 |
Jahr: | 26 November 2019 |
Umfang: | 23 S. |
Fussnoten: | Gesehen am 18.02.2020 |
Titel Quelle: | Enthalten in: Cancers |
Ort Quelle: | Basel : MDPI, 2009 |
Jahr Quelle: | 2019 |
Band/Heft Quelle: | 11(2019,12) Artikel-Nummer 1877, 23 Seiten |
ISSN Quelle: | 2072-6694 |
Abstract: | Background: Cancer patients are increasingly treated with alpha-particle-emitting radiopharmaceuticals. At the subcellular level, alpha particles induce densely spaced ionizations and molecular damage. Induction of DNA lesions, especially clustered DNA double-strand breaks (DSBs), threatens a cell’s survival. Currently, it is under debate to what extent the spatial topology of the damaged chromatin regions and the repair protein arrangements are contributing. Methods: Super-resolution light microscopy (SMLM) in combination with cluster analysis of single molecule signal-point density regions of DSB repair markers was applied to investigate the nano-structure of DNA damage foci tracks of Ra-223 in-solution irradiated leukocytes. Results: Alpha-damaged chromatin tracks were efficiently outlined by γ-H2AX that formed large (super) foci composed of numerous 60–80 nm-sized nano-foci. Alpha damage tracks contained 60–70% of all γ-H2AX point signals in a nucleus, while less than 30% of 53BP1, MRE11 or p-ATM signals were located inside γ-H2AX damage tracks. MRE11 and p-ATM protein fluorescent tags formed focal nano-clusters of about 20 nm peak size. There were, on average, 12 (±9) MRE11 nanoclusters in a typical γ-H2AX-marked alpha track, suggesting a minimal number of MRE11-processed DSBs per track. Our SMLM data suggest regularly arranged nano-structures during DNA repair in the damaged chromatin domain. |
DOI: | doi:10.3390/cancers11121877 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/cancers11121877 |
| Verlag: https://www.mdpi.com/2072-6694/11/12/1877 |
| DOI: https://doi.org/10.3390/cancers11121877 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | complex DNA damage |
| DNA repair |
| DSB focus substructure |
| high LET irradiation |
| Single Molecule Localization Microscopy (SMLM) |
K10plus-PPN: | 1690253126 |
Verknüpfungen: | → Zeitschrift |
Nanostructure of clustered DNA damage in leukocytes after in-solution irradiation with the alpha emitter Ra-223 / Scherthan, Harry [VerfasserIn]; 26 November 2019 (Online-Ressource)
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