| |  Online-Ressource |
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| Verfasst von: | Albert, Benjamin [VerfasserIn]  |
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| | Koš-Braun, Isabelle C. [VerfasserIn] |
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| | Henras, Anthony K [VerfasserIn] |
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| | Dez, Christophe [VerfasserIn] |
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| | Rueda, Maria Paula [VerfasserIn] |
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| | Zhang, Xu [VerfasserIn] |
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| | Gadal, Olivier [VerfasserIn] |
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| | Koš, Martin [VerfasserIn] |
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| | Shore, David [VerfasserIn] |
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| Titel: | A ribosome assembly stress response regulates transcription to maintain proteome homeostasis |
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| Verf.angabe: | Benjamin Albert, Isabelle C Kos-Braun, Anthony K Henras, Christophe Dez, Maria Paula Rueda, Xu Zhang, Olivier Gadal, Martin Kos, David Shore |
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| E-Jahr: | 2019 |
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| Jahr: | 2019 May 24 |
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| Umfang: | 24 S. |
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| Fussnoten: | Published online 2019 May 24 ; Gesehen am 25.02.2020 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 8(2019), Artikel-Nummer e45002, 24 Seiten |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | Ribosome biogenesis is a complex and energy-demanding process requiring tight coordination of ribosomal RNA (rRNA) and ribosomal protein (RP) production. Given the extremely high level of RP synthesis in rapidly growing cells, alteration of any step in the ribosome assembly process may impact growth by leading to proteotoxic stress. Although the transcription factor Hsf1 has emerged as a central regulator of proteostasis, how its activity is coordinated with ribosome biogenesis is unknown. Here, we show that arrest of ribosome biogenesis in the budding yeast Saccharomyces cerevisiae triggers rapid activation of a highly specific stress pathway that coordinately upregulates Hsf1 target genes and downregulates RP genes. Activation of Hsf1 target genes requires neo-synthesis of RPs, which accumulate in an insoluble fraction and presumably titrate a negative regulator of Hsf1, the Hsp70 chaperone. RP aggregation is also coincident with that of the RP gene activator Ifh1, a transcription factor that is rapidly released from RP gene promoters. Our data support a model in which the levels of newly synthetized RPs, imported into the nucleus but not yet assembled into ribosomes, work to continuously balance Hsf1 and Ifh1 activity, thus guarding against proteotoxic stress during ribosome assembly. |
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| DOI: | doi:10.7554/eLife.45002 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.7554/eLife.45002 |
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| | Verlag: https://doi.org/10.7554/eLife.45002 |
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| | DOI: https://doi.org/10.7554/eLife.45002 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | heat shock factor 1 |
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| | Ifh1 |
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| | protein aggregation |
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| | ribosomal protein |
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| | ribosome biogenesis |
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| | topoisomerase |
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| K10plus-PPN: | 1690969393 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ ribosome assembly stress response regulates transcription to maintain proteome homeostasis / Albert, Benjamin [VerfasserIn]; 2019 May 24 (Online-Ressource)
