Molecular diagnostics in pediatric brain tumors

• Verfasst von: Bächli, Heidi [VerfasserIn]
• Verfasst von: Ecker, Jonas [VerfasserIn]
• Verfasst von: Tilburg, Cornelis M. van [VerfasserIn]
• Verfasst von: Sturm, Dominik [VerfasserIn]
• Verfasst von: Selt, Florian [VerfasserIn]
• Verfasst von: Sahm, Felix [VerfasserIn]
• Verfasst von: Kölsche, Christian [VerfasserIn]
• Verfasst von: Grund, Kerstin [VerfasserIn]
• Verfasst von: Sutter, Christian [VerfasserIn]
• Verfasst von: Witt, Hendrik [VerfasserIn]
• Verfasst von: Herold-Mende, Christel [VerfasserIn]
• Verfasst von: Deimling, Andreas von [VerfasserIn]
• Verfasst von: Jones, David T. W. [VerfasserIn]
• Verfasst von: Pfister, Stefan [VerfasserIn]
• Verfasst von: Witt, Olaf [VerfasserIn]
• Verfasst von: Milde, Till [VerfasserIn]
• Titel: Molecular diagnostics in pediatric brain tumors
• Titelzusatz: impact on diagnosis and clinical decision-making - a selected case series
• Paralleltitel: Molekulare Diagnostik pädiatrischer Hirntumore$bEinfluss auf Diagnose und klinische Entscheidungen - eine ausgewählte Fallserie
• Verf.angabe: Heidi Bächli, Jonas Ecker, Cornelis van Tilburg, Dominik Sturm, Florian Selt, Felix Sahm, Christian Koelsche, Kerstin Grund, Christian Sutter, Torsten Pietsch, Hendrik Witt, Christel Herold-Mende, Andreas von Deimling, David Jones, Stefan Pfister, Olaf Witt, Till Milde
• E-Jahr: 2018
• Jahr: 11. Juli 2018
• Umfang: 9 S.
• Fussnoten: Gesehen am 02.03.2020
• Titel Quelle: Enthalten in: Klinische Pädiatrie
• Ort Quelle: Stuttgart : Thieme, 1980
• Jahr Quelle: 2018
• Band/Heft Quelle: 230(2018), 06, Seite 305-313
• ISSN Quelle: 1439-3824
• DOI: doi:10.1055/a-0637-9653
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1691314625

Abstract

Central nervous system (CNS) tumors account for the highest mortality among pediatric malignancies. Accurate diagnosis is essential for optimal clinical management. The increasing use of molecular diagnostics has opened up novel possibilities for more precise classification of CNS tumors. We here report a single-institutional collection of pediatric CNS tumor cases that underwent a refinement or a change of diagnosis after completion of molecular analysis that affected clinical decision-making including the application of molecularly informed targeted therapies. 13 pediatric CNS tumors were analyzed by conventional histology, immunohistochemistry, and molecular diagnostics including DNA methylation profiling in 12 cases, DNA sequencing in 8 cases and RNA sequencing in 3 cases. 3 tumors had a refinement of diagnosis upon molecular testing, and 6 tumors underwent a change of diagnosis. Targeted therapy was initiated in 5 cases. An underlying cancer predisposition syndrome was detected in 5 cases. Although this case series, retrospective and not population based, has its limitations, insight can be gained regarding precision of diagnosis and clinical management of the patients in selected cases. Accuracy of diagnosis was improved in the cases presented here by the addition of molecular diagnostics, impacting clinical management of affected patients, both in the first-line as well as in the follow-up setting. This additional information may support the clinical decision making in the treatment of challenging pediatric CNS tumors. Prospective testing of the clinical value of molecular diagnostics is currently underway.