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Status: Bibliographieeintrag

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Verfasst von:Pandey, Gunjan [VerfasserIn]   i
 Westhoff, Jens [VerfasserIn]   i
 Schaefer, Franz [VerfasserIn]   i
 Gehrig, Jochen [VerfasserIn]   i
Titel:A smart imaging workflow for organ-specific screening in a cystic kidney zebrafish disease model
Verf.angabe:Gunjan Pandey, Jens H. Westhoff, Franz Schaefer and Jochen Gehrig
E-Jahr:2019
Jahr:14 March 2019
Umfang:16 S.
Fussnoten:Gesehen am 06.03.2020
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2019
Band/Heft Quelle:20(2019,6) Artikel-Nummer 1290, 16 Seiten
ISSN Quelle:1422-0067
 1661-6596
Abstract:The zebrafish is being increasingly used in biomedical research and drug discovery to conduct large-scale compound screening. However, there is a lack of accessible methodologies to enable automated imaging and scoring of tissue-specific phenotypes at enhanced resolution. Here, we present the development of an automated imaging pipeline to identify chemical modifiers of glomerular cyst formation in a zebrafish model for human cystic kidney disease. Morpholino-mediated knockdown of intraflagellar transport protein Ift172 in Tg(wt1b:EGFP) embryos was used to induce large glomerular cysts representing a robustly scorable phenotypic readout. Compound-treated embryos were consistently aligned within the cavities of agarose-filled microplates. By interfacing feature detection algorithms with automated microscopy, a smart imaging workflow for detection, centring and zooming in on regions of interests was established, which enabled the automated capturing of standardised higher resolution datasets of pronephric areas. High-content screening datasets were processed and analysed using custom-developed heuristic algorithms implemented in common open-source image analysis software. The workflow enables highly efficient profiling of entire compound libraries and scoring of kidney-specific morphological phenotypes in thousands of zebrafish embryos. The demonstrated toolset covers all the aspects of a complex whole organism screening assay and can be adapted to other organs, specimens or applications.
DOI:doi:10.3390/ijms20061290
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/ijms20061290
 Volltext: https://www.mdpi.com/1422-0067/20/6/1290
 DOI: https://doi.org/10.3390/ijms20061290
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:automated imaging
 high-content screening
 image analysis
 zebrafish
K10plus-PPN:1691850519
Verknüpfungen:→ Zeitschrift

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