Toward an ensemble view of chromatosome structure

• Verfasst von: Öztürk, Mehmet Ali [VerfasserIn]
• Verfasst von: Cojocaru, Vlad [VerfasserIn]
• Verfasst von: Wade, Rebecca C. [VerfasserIn]
• Titel: Toward an ensemble view of chromatosome structure
• Titelzusatz: a paradigm shift from one to many
• Verf.angabe: Mehmet Ali Öztürk, Vlad Cojocaru, and Rebecca C. Wade
• E-Jahr: 2018
• Jahr: August 7, 2018
• Umfang: 8 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 10.03.2020
• Titel Quelle: Enthalten in: Structure
• Ort Quelle: London [u.a.] : Elsevier Science, 1993
• Jahr Quelle: 2018
• Band/Heft Quelle: 26(2018), 8, Seite 1050-1057
• ISSN Quelle: 1878-4186
• DOI: doi:10.1016/j.str.2018.05.009
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: chromatin
• Sach-SW: chromatosome
• Sach-SW: cryo-EM
• Sach-SW: linker histone
• Sach-SW: macromolecular dynamics
• Sach-SW: macromolecular structure
• Sach-SW: NMR
• Sach-SW: nucleosome
• Sach-SW: X-ray crystallography
• K10plus-PPN: 1692195816

Abstract

There is renewed interest in linker histone (LH)—nucleosome binding and how LHs influence eukaryotic DNA compaction. For a long time, the goal was to uncover “the structure of the chromatosome,” but recent studies of LH-nucleosome complexes have revealed an ensemble of structures. Notably, the reconstituted LH-nucleosome complexes used in experiments rarely correspond to the sequence combinations present in organisms. For a full understanding of the determinants of the distribution of the chromatosome structural ensemble, studies must include a complete description of the sequences and experimental conditions used, and be designed to enable systematic evaluation of sequence and environmental effects.