Tuning RGD motif and hyaluronan density to study integrin binding

• Verfasst von: Zapp, Cornelia [VerfasserIn]
• Verfasst von: Minsky, Burcu Baykal [VerfasserIn]
• Verfasst von: Böhm, Heike [VerfasserIn]
• Titel: Tuning RGD motif and hyaluronan density to study integrin binding
• Verf.angabe: Cornelia Zapp, Burcu B. Minsky and Heike Boehm
• E-Jahr: 2018
• Jahr: 07 August 2018
• Umfang: 8 S.
• Teil: volume:9
• Teil: year:2018
• Teil: extent:8
• Fussnoten: Gesehen am 11.03.2020
• Titel Quelle: Enthalten in: Frontiers in physiology
• Ort Quelle: Lausanne : Frontiers Research Foundation, 2007
• Jahr Quelle: 2018
• Band/Heft Quelle: 9(2018) Artikel-Nummer 1022, 8 Seiten
• ISSN Quelle: 1664-042X
• DOI: doi:10.3389/fphys.2018.01022
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cell Adhesion
• Sach-SW: hyaluronan
• Sach-SW: Integrins
• Sach-SW: proteoliposomes
• Sach-SW: QCM-D
• K10plus-PPN: 1692284045

Abstract

Well-controlled surfaces with immobilized substrates enable novel approaches to investigate specific aspects of biological processes related to cell adhesion or motility. A subset of integrins, cellular transmembrane glycoproteins, recognize the evolutionarily conserved tripeptide sequence RGD, and anchor cells to their surrounding proteins as well as mediate bidirectional signaling. In this study, the main question was how co-presentation of hyaluronan, an essential component of the extracellular matrix, and the RGD motif affect integrin binding. We report a method to prepare self-assembled monolayers on gold surfaces, co-presenting the cell adhesive RGD motif and small hyaluronan molecules, to investigate integrin containing proteoliposome binding. This technique enables an independent adjustment of the RGD motif and hyaluronan density while maintaining a passivating background.. Layer formation and subsequent interactions with αIIbβ3 integrins, which are reconstituted in liposomes, was monitored by label-free quartz crystal microbalance with dissipation monitoring (QCM-D). Exceeding a critical RGD motif density of 40% results in enhanced binding of proteoliposomes. Co-presentation studies with varying hyaluronan and constant RGD motif density demonstrate that marginal amounts of hyaluronan are sufficient to prevent integrin binding. These findings are of specific importance in relation to cancer cell microenvironment, which show highly enriched hyaluronan in the surrounding extracellular matrix to reduce adhesion properties.