A potential new metabolite of gamma-hydroxybutyrate

• Verfasst von: Hanisch, Stephanie [VerfasserIn]
• Verfasst von: Stachel, Nicole [VerfasserIn]
• Verfasst von: Skopp, Gisela [VerfasserIn]
• Titel: A potential new metabolite of gamma-hydroxybutyrate
• Titelzusatz: sulfonated gamma-hydroxybutyric acid
• Verf.angabe: Stephanie Hanisch, Nicole Stachel, Gisela Skopp
• Jahr: 2016
• Jahr des Originals: 2015
• Umfang: 4 S.
• Fussnoten: Published online: 26 July 2015 ; Gesehen am 12.03.2020
• Titel Quelle: Enthalten in: International journal of legal medicine
• Ort Quelle: Berlin : Springer, 1922
• Jahr Quelle: 2016
• Band/Heft Quelle: 130(2016), 2, Seite 411-414
• ISSN Quelle: 1437-1596
• DOI: doi:10.1007/s00414-015-1235-x
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 169238872X

Abstract

Detection of gamma-hydroxybutyric acid (GHB) became crucial in many clinical and forensic settings due to its increasing use for recreational purposes and drug-facilitated sexual assault. Its narrow window of detection of about 3-12 h in urine represents a major problem. Analogous to ethyl glucuronide, the recently identified GHB-glucuronide exhibits a longer window of detection than the parent drug. It appeared reasonable that a sulfonated metabolite of GHB (GHB-SUL) will also be formed. Due to the lack of an appropriate standard, GHB was incubated with a human liver cytosolic fraction to produce GHB-SUL. Following development of a liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay to measure GHB and GHB-SUL, authentic urine samples (n = 5) were tested for GHB-SUL. These investigations revealed detectable signals of both GHB and GHB-SUL, strongly indicating that GHB is not only glucuronidated but also sulfonated. Given that sulfonated metabolites generally have longer half-life times than the corresponding free drugs, GHB-SUL may serve as a biomarker of GHB misuse along with its glucuronide.