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Status: Bibliographieeintrag

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Verfasst von:Hartwig, Vanessa [VerfasserIn]   i
 Dewidar, Bedair [VerfasserIn]   i
 Lin, Tao [VerfasserIn]   i
 Dropmann, Anne [VerfasserIn]   i
 Ganss, Christoph [VerfasserIn]   i
 Kluth, Mark Andreas [VerfasserIn]   i
 Tappenbeck, Nils [VerfasserIn]   i
 Tietze, Lysann [VerfasserIn]   i
 Christ, Bruno [VerfasserIn]   i
 Frank, Markus [VerfasserIn]   i
 Vogelmann, Roger [VerfasserIn]   i
 Ebert, Matthias [VerfasserIn]   i
 Dooley, Steven [VerfasserIn]   i
Titel:Human skin-derived ABCB5+ stem cell injection improves liver disease parameters in Mdr2KO mice
Verf.angabe:Vanessa Hartwig, Bedair Dewidar, Tao Lin, Anne Dropmann, Christoph Ganss, Mark Andreas Kluth, Nils Tappenbeck, Lysann Tietze, Bruno Christ, Markus Frank, Roger Vogelmann, Matthias Philip Alexander Ebert, Steven Dooley
E-Jahr:2019
Jahr:21 August 2019
Umfang:16 S.
Fussnoten:Gesehen am 13.03.2020 ; Im Titel ist "+" hochgestellt
Titel Quelle:Enthalten in: Archives of toxicology
Ort Quelle:Berlin : Springer, 1930
Jahr Quelle:2019
Band/Heft Quelle:93(2019), 9, Seite 2645-2660
ISSN Quelle:1432-0738
Abstract:Although liver transplantation is a potential effective cure for patients with end-stage liver diseases, this strategy has several drawbacks including high cost, long waiting list, and limited availability of liver organs. Therefore, stem cell-based therapy is presented as an alternative option, which showed promising results in animal models of acute and chronic liver injuries. ABCB5+ cells isolated from skin dermis represent an easy accessible and expandable source of homogenous stem cell populations. In addition, ABCB5+ cells showed already promising results in the treatment of corneal and skin injury. To date, the effect of these cells on liver injury is still unknown. In the current study, sixteen weeks old Mdr2KO mice were i.v. injected with 500,000 ABCB5+ cells using different experimental setups. The effects of cellular therapy on inflammation, fibrosis, apoptosis, and proliferation were analyzed in the collected liver tissues. Toxicity of ABCB5+ cells was additionally investigated in mice with partial liver resection. In vitro, the fibrosis- and inflammatory-modulating effects of supernatant from ABCB5+ cells were examined in the human hepatic stellate cell line (LX-2). Cell injections into fibrotic Mdr2KO mice as well as into mice upon partial liver resection have no signs of toxicity with regard to cell transformation, cellular damage, fibrosis or inflammation as compared to controls. We next investigated the effects of ABCB5+ cells on established biliary liver fibrosis in the Mdr2KO mice. ABCB5+ cells to some extent influenced the shape of the liver inflammatory response and significantly reduced the amount of collagen deposition, as estimated from quantification of sirius red staining. Furthermore, reduced apoptosis and enhanced death compensatory proliferation resulted from ABCB5+ cell transformation. The stem cells secreted several trophic factors that activated TGF-β family signaling in cultured LX-2 hepatic stellate cells (HSCs), therewith shaping cell fate to an αSMAhigh, Vimentinlow phenotype. Taken together, ABCB5+ cells can represent a safe and feasible strategy to support liver regeneration and to reduce liver fibrosis in chronic liver diseases.
DOI:doi:10.1007/s00204-019-02533-3
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1007/s00204-019-02533-3
 DOI: https://doi.org/10.1007/s00204-019-02533-3
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:ABCB5
 Cell therapy
 Liver fibrosis
 Mdr2KO
 Mesenchymal stromal cells
K10plus-PPN:1692461680
Verknüpfungen:→ Zeitschrift

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