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| Verfasst von: | Sottas, Valentin [VerfasserIn]  |
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| | Wahl, Carl-Mattheis [VerfasserIn] |
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| | Trache, Cristian [VerfasserIn] |
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| | Bartolf-Kopp, Michael [VerfasserIn] |
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| | Cambridge, Sidney [VerfasserIn] |
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| | Hecker, Markus [VerfasserIn] |
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| | Ullrich, Nina D. [VerfasserIn] |
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| Titel: | Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression |
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| Verf.angabe: | Valentin Sottas, Carl-Mattheis Wahl, Mihnea C. Trache, Michael Bartolf-Kopp, Sidney Cambridge, Markus Hecker, Nina D. Ullrich |
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| E-Jahr: | 2018 |
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| Jahr: | 16 May 2018 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 30.03.2020 |
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| Titel Quelle: | Enthalten in: Journal of molecular and cellular cardiology |
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| Ort Quelle: | New York, NY [u.a.] : Elsevier, 1970 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 120(2018), Seite 31-41 |
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| ISSN Quelle: | 1095-8584 |
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| Abstract: | Abstract: The therapeutic potential of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is limited by immature functional features including low impulse propagation and reduced cell excitability. Key players regulating electrical activity are voltage-gated Na<sup>+</sup> channels (Na<sub>v</sub>1.5) and gap junctions built from connexin-43 (Cx43). Here we tested the hypothesis that enhanced Cx43 expression increases intercellular coupling and influences excitability by modulating Na<sub>v</sub>1.5. Using transgenic approaches, Cx43 and Na<sub>v</sub>1.5 localization and cell coupling were studied by confocal imaging. Na<sub>v</sub>1.5 currents and action potentials (APs) were measured using the patch-clamp technique. Enhanced sarcolemmal Cx43 expression significantly improved intercellular coupling and accelerated dye transfer kinetics. Furthermore, Cx43 modulated Na<sub>v</sub>1.5 function leading to significantly higher current and enhanced AP upstroke velocities, thereby improving electrical activity as measured by microelectrode arrays. These findings suggest a mechanistic link between cell coupling and excitability controlled by Cx43 expression in iPSC-CMs. Therefore, we propose Cx43 as novel molecular target for improving electrical properties of iPSC-CMs to match the functional properties of native myocytes. |
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| DOI: | doi:10.1016/j.yjmcc.2018.05.010 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.yjmcc.2018.05.010 |
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| | Volltext: https://www.jmmc-online.com/article/S0022-2828(18)30171-8/abstract |
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| | DOI: https://doi.org/10.1016/j.yjmcc.2018.05.010 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1693473941 |
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| Verknüpfungen: | → Zeitschrift |
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Improving electrical properties of iPSC-cardiomyocytes by enhancing Cx43 expression / Sottas, Valentin [VerfasserIn]; 16 May 2018 (Online-Ressource)
