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Verfasst von:Esmaeilzadeh, Majid [VerfasserIn]   i
 Sadeghi, Mahmoud [VerfasserIn]   i
 Heissler, Hans E. [VerfasserIn]   i
 Galmbacher, Roland [VerfasserIn]   i
 Majlesara, Ali [VerfasserIn]   i
 Al-Afif, Shadi [VerfasserIn]   i
 Mehrabi, Arianeb [VerfasserIn]   i
Titel:Experimental rat model for brain death induction and kidney transplantation
Verf.angabe:Majid Esmaeilzadeh, Mahmoud Sadeghi, Hans E. Heissler, Roland Galmbacher, Ali Majlesara, Shadi Al-Afif, Arianeb Mehrabi
Jahr:2020
Umfang:6 S.
Teil:volume:33
 year:2020
 number:2
 pages:141-146
 extent:6
Fussnoten:Published online: 18 Oct 2018 ; Gesehen am 01.04.2020
Titel Quelle:Enthalten in: Journal of investigative surgery
Ort Quelle:Abingdon : Taylor & Francis Group, 1988
Jahr Quelle:2020
Band/Heft Quelle:33(2020), 2, Seite 141-146
ISSN Quelle:1521-0553
Abstract:Background: Experimental animal research has been pivotal in developing clinical kidney transplantation (KTx). One donor-associated risk factor with negative affect of transplantation outcome is brain death (BD). Many rat models for BD and KTx have been developed in the last decade, but no surgical guidelines have been developed for these models. Here, we describe a surgical technique for BD induction and the cuff technique for experimental KTx in rats.Methods: After intubation and mechanically ventilation of sixteen healthy adult male Sprague-Dawley rats were induction of BD performed. Animals were kept hemodynamically stable for eight hours. Then, the kidney was prepared and perfused with standard histidine-tryptophan-ketoglutarate solution. After explantation, grafts were immediately implanted in recipients using the cuff technique and reperfused. After 2 h of observation, animals were sacrificed by intravenous administration of potassium chloride.Results: In the early phase of BD, heart rate increased and mean arterial pressure decreased. Partial variations were observed in O2 partial pressure, O2 saturation, and HCO3. During the 2-h observation phase, all transplanted kidneys were sufficiently perfused macroscopically. There was no hyperacute rejection.Conclusions: It is feasible to observe BD for 8 h with maintained circulation in small experimental settings. The cuff technique for KTx is simple, the complication rate is low, and the warm ischemia time is short, therefore, this could be a suitable technique for KTx in the rat model.
DOI:doi:10.1080/08941939.2018.1480677
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1080/08941939.2018.1480677
 DOI: https://doi.org/10.1080/08941939.2018.1480677
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:brain death
 experimental research
 feasibility
 graft
 kidney transplantation
 rat model
K10plus-PPN:1693762625
Verknüpfungen:→ Zeitschrift

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