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Verfasst von:Riedel, Christiane Maria [VerfasserIn]   i
 Chen, Hann-Wei [VerfasserIn]   i
 Reichart, Ursula [VerfasserIn]   i
 Lamp, Benjamin [VerfasserIn]   i
 Laketa, Vibor [VerfasserIn]   i
 Rümenapf, Till [VerfasserIn]   i
Titel:Real time analysis of bovine viral diarrhea virus (BVDV) infection and its dependence on bovine CD46
Verf.angabe:Christiane Riedel, Hann-Wei Chen, Ursula Reichart, Benjamin Lamp, Vibor Laketa and Till Rümenapf
E-Jahr:2020
Jahr:17 January 2020
Umfang:13 S.
Fussnoten:Gesehen am 02.04.2020
Titel Quelle:Enthalten in: Viruses
Ort Quelle:Basel : MDPI, 2009
Jahr Quelle:2020
Band/Heft Quelle:12(2020,1) Artikel-Nummer 116, 13 Seiten
ISSN Quelle:1999-4915
Abstract:Virus attachment and entry is a complex interplay of viral and cellular interaction partners. Employing bovine viral diarrhea virus (BVDV) encoding an mCherry-E2 fusion protein (BVDVE2-mCherry), being the first genetically labelled member of the family Flaviviridae applicable for the analysis of virus particles, the early events of infection—attachment, particle surface transport, and endocytosis—were monitored to better understand the mechanisms underlying virus entry and their dependence on the virus receptor, bovine CD46. The analysis of 801 tracks on the surface of SK6 cells inducibly expressing fluorophore labelled bovine CD46 (CD46fluo) demonstrated the presence of directed, diffusive, and confined motion. 26 entry events could be identified, with the majority being associated with a CD46fluo positive structure during endocytosis and occurring more than 20 min after virus addition. Deletion of the CD46fluo E2 binding domain (CD46fluo∆E2bind) did not affect the types of motions observed on the cell surface but resulted in a decreased number of observable entry events (2 out of 1081 tracks). Mean squared displacement analysis revealed a significantly increased velocity of particle transport for directed motions on CD46fluo∆E2bind expressing cells in comparison to CD46fluo. These results indicate that the presence of bovine CD46 is only affecting the speed of directed transport, but otherwise not influencing BVDV cell surface motility. Instead, bovine CD46 seems to be an important factor during uptake, suggesting the presence of additional cellular proteins interacting with the virus which are able to support its transport on the virus surface.
DOI:doi:10.3390/v12010116
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/v12010116
 Volltext: https://www.mdpi.com/1999-4915/12/1/116
 DOI: https://doi.org/10.3390/v12010116
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:<i>Pestivirus</i>
 attachment
 BVDV
 CD46
 life cell imaging
 surface transport
K10plus-PPN:1693803240
Verknüpfungen:→ Zeitschrift

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